About: HIV-1 matrix protein repositioning in nucleocapsid region fails to confer virus-like particle assembly

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About: HIV-1 matrix protein repositioning in nucleocapsid region fails to confer virus-like particle assembly Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	HIV-1 matrix protein repositioning in nucleocapsid region fails to confer virus-like particle assembly
Creator	Chang, Ching-Yuan Chang, Yu-Fen Huang, Kuo-Jung Tseng, Ying-Tzu Wang, Chin-Tien »more»
source	Elsevier; Medline; PMC
abstract	Abstract The HIV-1 matrix (MA) protein is similar to nucleocapsid (NC) proteins in its propensity for self-interaction and association with RNA. Here we report on our finding that replacing MA with NC results in the production of wild type (wt)-level RNA and virus-like particles (VLPs). In contrast, constructs containing MA as a substitute for NC are markedly defective in VLP production and form virions with lower densities than wt, even though their RNA content is over 50% that of wt level. We also noted that a ΔMN mutant lacking both MA and NC produces a relatively higher amount of VLPs than those in which MA was substituted for NC. Although ΔMN contains approximately 30% the RNA of wt, it still exhibits virion densities equal (or very similar) to those of wt. The data suggest that neither NC nor RNA are major virion density determinants. Furthermore, we noted that NC(ZIP)—a NC replacement with a leucine zipper dimerization motif—produces VLPs as efficiently as wt. However, the markedly reduced assembly efficiency of NC(ZIP) is associated with the formation of VLPs with densities slightly lower than those of wt following MA removal, suggesting that (a) MA is required to help the inserted leucine zipper motif perform efficient Gag multimerization, and (b) MA plays a role in the virus assembly process.
has issue date	2008-08-15(xsd:dateTime)
bibo:doi	10.1016/j.virol.2008.05.010
bibo:pmid	18550141
has license	els-covid
sha1sum (hex)	f774a2decc541f4bf1265b269e37ae9c0753a321
schema:url	https://doi.org/10.1016/j.virol.2008.05.010
resource representing a document's title	HIV-1 matrix protein repositioning in nucleocapsid region fails to confer virus-like particle assembly
has PubMed Central identifier	PMC7103396
has PubMed identifier	18550141
schema:publication	Virology
resource representing a document's body	covid:f774a2decc541f4bf1265b269e37ae9c0753a321#body_text
is schema:about of	named entity 'VLP' named entity 'virions' named entity 'confer' covid:arg/f774a2decc541f4bf1265b269e37ae9c0753a321 named entity 'defective' »more»

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