About: Induction of hairy roots by Agrobacterium rhizogenes-mediated transformation of spine gourd (Momordica dioica Roxb. ex. willd) for the assessment of phenolic compounds and biological activities

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About: Induction of hairy roots by Agrobacterium rhizogenes-mediated transformation of spine gourd (Momordica dioica Roxb. ex. willd) for the assessment of phenolic compounds and biological activities Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Induction of hairy roots by Agrobacterium rhizogenes-mediated transformation of spine gourd (Momordica dioica Roxb. ex. willd) for the assessment of phenolic compounds and biological activities
Creator	Chung, Ill-Min Rekha, Kaliyaperumal Thiruvengadam, Muthu
source	Elsevier; Medline; PMC
abstract	Abstract An efficient protocol for hairy root induction of spine gourd (Momordica dioica) was established using Agrobacterium rhizogenes (KCTC 2703). This study evaluates the phenolic compound production, antioxidant and antimicrobial (antibacterial, antifungal and antiviral) activities of transgenic hairy root cultures in M. dioica . Hairy roots were induced from leaves, petiole, and internodal explants. Molecular analysis of PCR and gene sequencing using specific primers of rolC and aux1 revealed T-DNA integration in the hairy root clones and RT-PCR analysis confirmed the expression of hairy root inducible genes (rolC and aux1). The greatest biomass accumulation of hairy roots on MS liquid medium supplemented with 3% sucrose was observed at 22 days. Ultra-HPLC was used to compare the individual phenolic compound contents of transgenic and non-transgenic roots. Moreover, transgenic hairy roots efficiently produced several phenolic compounds, such as flavonols, hydroxycinnamic acid and hydroxybenzoic acid derivatives. The total phenolic, flavonoid contents and biological (antioxidant, antibacterial, antifungal and antiviral) activities were higher in hairy roots compared to non-transformed roots. These results demonstrate the greater potentiality of M. dioica hairy root cultures for the production of valuable phenolic compounds and for studies of their biological activity.
has issue date	2016-01-26(xsd:dateTime)
bibo:doi	10.1016/j.scienta.2015.11.035
bibo:pmid	32287883
has license	els-covid
sha1sum (hex)	e7398840f44a37ae50c0c5da49c4b423a070b271
schema:url	https://doi.org/10.1016/j.scienta.2015.11.035
resource representing a document's title	Induction of hairy roots by Agrobacterium rhizogenes-mediated transformation of spine gourd (Momordica dioica Roxb. ex. willd) for the assessment of phenolic compounds and biological activities
has PubMed Central identifier	PMC7116902
has PubMed identifier	32287883
schema:publication	Scientia Horticulturae
resource representing a document's body	covid:e7398840f44a37ae50c0c5da49c4b423a070b271#body_text
is schema:about of	named entity 'ACTIVITIES' named entity 'GOURD' named entity 'HYDROXYCINNAMIC ACID' named entity 'CULTURES' named entity 'MOLECULAR ANALYSIS' »more»

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