About: Mosquito cell-derived West Nile virus replicon particles mimic arbovirus inoculum and have reduced spread in mice

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Mosquito cell-derived West Nile virus replicon particles mimic arbovirus inoculum and have reduced spread in mice
Creator	Bernard, Kristen Boylan, Brendan Carlson, Tim Moreira, Fernando
source	PMC
abstract	Half of the human population is at risk of infection by an arthropod-borne virus. Many of these arboviruses, such as West Nile, dengue, and Zika viruses, infect humans by way of a bite from an infected mosquito. This infectious inoculum is insect cell-derived giving the virus particles distinct qualities not present in secondary infectious virus particles produced by infected vertebrate host cells. The insect cell-derived particles differ in the glycosylation of virus structural proteins and the lipid content of the envelope, as well as their induction of cytokines. Thus, in order to accurately mimic the inoculum delivered by arthropods, arboviruses should be derived from arthropod cells. Previous studies have packaged replicon genome in mammalian cells to produce replicon particles, which undergo only one round of infection, but no studies exist packaging replicon particles in mosquito cells. Here we optimized the packaging of West Nile virus replicon genome in mosquito cells and produced replicon particles at high concentration, allowing us to mimic mosquito cell-derived viral inoculum. These particles were mature with similar genome equivalents-to-infectious units as full-length West Nile virus. We then compared the mosquito cell-derived particles to mammalian cell-derived particles in mice. Both replicon particles infected skin at the inoculation site and the draining lymph node by 3 hours post-inoculation. The mammalian cell-derived replicon particles spread from the site of inoculation to the spleen and contralateral lymph nodes significantly more than the particles derived from mosquito cells. This in vivo difference in spread of West Nile replicons in the inoculum demonstrates the importance of using arthropod cell-derived particles to model early events in arboviral infection and highlights the value of these novel arthropod cell-derived replicon particles for studying the earliest virus-host interactions for arboviruses.
has issue date	2017-02-10(xsd:dateTime)
bibo:doi	10.1371/journal.pntd.0005394
bibo:pmid	28187142
has license	cc-by
sha1sum (hex)	e7124df42078edba08ccba1b033dffb0a36f6305
schema:url	https://doi.org/10.1371/journal.pntd.0005394
resource representing a document's title	Mosquito cell-derived West Nile virus replicon particles mimic arbovirus inoculum and have reduced spread in mice
has PubMed Central identifier	PMC5322982
has PubMed identifier	28187142
schema:publication	PLoS Negl Trop Dis
resource representing a document's body	covid:e7124df42078edba08ccba1b033dffb0a36f6305#body_text
is schema:about of	named entity 'cytokines' named entity 'packaging' named entity 'genome' named entity 'mature' named entity 'draining' »more»

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