About: Effect of pressure on refolding of recombinant pentameric cholera toxin B

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Effect of pressure on refolding of recombinant pentameric cholera toxin B
Creator	Chura-Chambi, R Farinha-Arcieri, L Guimarães, J Ho, P Lemke, L »more»
source	Elsevier; Medline; PMC
abstract	Abstract The production of recombinant proteins is an essential tool for the expansion of modern biological research and biotechnology. The expression of heterologous proteins in Escherichia coli often results in an incomplete folding process that leads to the accumulation of inclusion bodies (IB), aggregates that hold a certain degree of native-like secondary structure. High hydrostatic pressure (HHP) impairs intermolecular hydrophobic and electrostatic interactions, leading to dissociation of aggregates under non-denaturing conditions and is therefore a useful tool to solubilize proteins for posterior refolding. Cholera toxin (CT) is composed of a non-toxic pentamer of B subunits (CTB), a useful adjuvant in vaccines, and a toxic subunit A (CTA). We studied the process of refolding of CTB using HHP. HHP was shown to be effective for dissociation of CTB monomers from IB. Posterior incubation at atmospheric pressure of concentrated CTB (1mg/ml) is necessary for the association of the monomers. Pentameric CTB was obtained when suspensions of CTB IB were compressed at 2.4kbar for 16h in the presence of Tween 20 and incubated at 1bar for 120h. Soluble and biologically active pentameric CTB was obtained, with a yield of 213mg CTB/liter of culture. The experience gained in this study can be important to improve the refolding of proteins with quaternary structure.
has issue date	2014-03-10(xsd:dateTime)
bibo:doi	10.1016/j.jbiotec.2013.12.006
bibo:pmid	24445168
has license	els-covid
sha1sum (hex)	e5dd23fb188c4db66feb00bee259fae34d6c9ef9
schema:url	https://doi.org/10.1016/j.jbiotec.2013.12.006
resource representing a document's title	Effect of pressure on refolding of recombinant pentameric cholera toxin B
has PubMed Central identifier	PMC7114129
has PubMed identifier	24445168
schema:publication	Journal of Biotechnology
resource representing a document's body	covid:e5dd23fb188c4db66feb00bee259fae34d6c9ef9#body_text
is schema:about of	named entity 'tool' named entity 'biologically active' named entity 'concentrated' named entity 'subunits' named entity 'recombinant' »more»

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