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About:
Presence and vitality of SARS-CoV-2 virus in wastewaters and rivers
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covidontheweb.inria.fr
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Academic Article
research paper
schema:ScholarlyArticle
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type
Academic Article
research paper
schema:ScholarlyArticle
isDefinedBy
Covid-on-the-Web dataset
title
Presence and vitality of SARS-CoV-2 virus in wastewaters and rivers
Creator
Gismondo, Maria
Mancon, Alessandro
Mileto, Davide
Comandatore, Francesco
Gigantiello, Anna
»more»
source
MedRxiv
abstract
Wastewater-based epidemiology has been proposed to monitor the diffusion and trend of SARS-CoV-2 pandemic. In the present study, raw and treated samples from three wastewater treatment plants, and two river samples characterized the Milano Metropolitan Area, Italy, were surveyed for SARS-CoV-2 RNA positivity to real time PCR and infectiveness. Moreover, whole genome sequencing and phylogenetic analysis of isolated strains was performed. Raw wastewater samples resulted positive to PCR amplification, while treated water samples were always negative (four and two samples, respectively, sampled in two dates). Moreover, the rate of positivity in raw wastewater samples decreased after eight days, in congruence with the epidemiological trend estimated for the interested provinces. Virus infectiveness was always not significant, indicating the effectiveness of wastewater treatments, or the natural decay of viral vitality, which implied the absence of significant risk of infection from wastewaters. Samples from receiving rivers (two sites, sampled in the same dates as wastewaters) showed in some cases a positivity to PCR amplification, probably due to non-treated discharges, or the combined sewage overflows. Nevertheless, also for rivers vitality was negligible, indicating the absence of sanitary risks. Phylogenetic analysis of genome indicated that the isolated virus belongs to the most spread strain present in Europe and similar to another strain found in Lombardy.
has issue date
2020-05-05
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bibo:doi
10.1101/2020.05.01.20086009
has license
medrxiv
sha1sum (hex)
dde8f534b43dd44041a4820eee0849e70842ae28
schema:url
https://doi.org/10.1101/2020.05.01.20086009
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Presence and vitality of SARS-CoV-2 virus in wastewaters and rivers
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covid:dde8f534b43dd44041a4820eee0849e70842ae28#body_text
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named entity 'positivity'
named entity 'SARS-CoV-2'
named entity 'wastewaters'
named entity 'PHYLOGENETIC ANALYSIS'
named entity 'ITALY'
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