About: Nucleotide bearing benzylidene‐tetrahydroxanthylium near‐IR fluorophore for sensing DNA replication, secondary structures and interactions

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About: Nucleotide bearing benzylidene‐tetrahydroxanthylium near‐IR fluorophore for sensing DNA replication, secondary structures and interactions Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Nucleotide bearing benzylidene‐tetrahydroxanthylium near‐IR fluorophore for sensing DNA replication, secondary structures and interactions
Creator	Pohl, Radek Hocek, Michal Kraus, Tomáš Kuba, Miroslav
source	PMC
abstract	Thymidine triphosphate bearing benzylidene‐tetrahydroxanthylium near‐IR fluorophore linked to the 5‐methyl group via triazole was synthesized through CuAAC reaction and was used for polymerase synthesis of labelled DNA probes. The fluorophore lights up upon incorporation to DNA (up to 348‐times) presumably due to interactions in major groove and the fluorescence further increases in single‐stranded oligonucleotide. The labelled dsDNA senses binding of small molecules and proteins by strong decrease of fluorescence. The nucleotide was used as a light‐up building block in real‐time PCR for detection of SARS‐CoV‐2 virus.
has issue date	2020-07-07(xsd:dateTime)
bibo:doi	10.1002/chem.202003192
bibo:pmid	32633433
has license	no-cc
sha1sum (hex)	dc60d0d00fae997df65ee8e490d96eb5ae4558dd
schema:url	https://doi.org/10.1002/chem.202003192
resource representing a document's title	Nucleotide bearing benzylidene‐tetrahydroxanthylium near‐IR fluorophore for sensing DNA replication, secondary structures and interactions
has PubMed Central identifier	PMC7361531
has PubMed identifier	32633433
schema:publication	Chemistry
resource representing a document's body	covid:dc60d0d00fae997df65ee8e490d96eb5ae4558dd#body_text
is schema:about of	named entity 'single-stranded' named entity 'probes' named entity 'SARS-CoV-2' named entity 'OLIGONUCLEOTIDE' named entity 'MAJOR GROOVE' »more»

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