About: Simultaneous detection and differentiation of canine parvovirus and feline parvovirus by high resolution melting analysis

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About: Simultaneous detection and differentiation of canine parvovirus and feline parvovirus by high resolution melting analysis Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Simultaneous detection and differentiation of canine parvovirus and feline parvovirus by high resolution melting analysis
Creator	Cao, Zhigang Cheng, Shipeng Cheng, Yuening Li, Shuang Lin, Peng »more»
source	Medline; PMC
abstract	BACKGROUND: Canine parvovirus (CPV) and feline parvovirus (FPV) are causative agents of diarrhea in dogs and cats, which manifests as depression, vomiting, fever, loss of appetite, leucopenia, and diarrhea in young animals. CPV and FPV can single or mixed infect cats and cause disease. To diagnose sick animals effectively, an effective virus diagnostic and genome typing method with high sensitivity and specificity is required. RESULTS: In this study, a conserved segment containing one SNP A4408C of parvovirus was used for real-time PCR amplification. Subsequently, data were auto-analyzed and plotted using Applied Biosystems® High Resolution Melt Software v3.1. Results showed that CPV and FPV can be detected simultaneously in a single PCR reaction. No cross-reactions were observed with canine adenovirus, canine coronavirus, and canine distemper virus. The assay had a detection limit of 4.2 genome copies of CPV and FPV. A total of 80 clinical samples were subjected to this assay, as well as to conventional PCR-sequence assay and virus isolation. Results showed that the percentage of agreement of the assay and other methods are high. CONCLUSIONS: In short, we have developed a diagnostic test for the accurate detection and differentiation of CPV and FPV in fecal samples, which is also cost effective.
has issue date	2019-05-10(xsd:dateTime)
bibo:doi	10.1186/s12917-019-1898-5
bibo:pmid	31077252
has license	cc-by
sha1sum (hex)	ce2f322118541b9901f218ce6e49177e5d25dd6c
schema:url	https://doi.org/10.1186/s12917-019-1898-5
resource representing a document's title	Simultaneous detection and differentiation of canine parvovirus and feline parvovirus by high resolution melting analysis
has PubMed Central identifier	PMC6511188
has PubMed identifier	31077252
schema:publication	BMC Vet Res
resource representing a document's body	covid:ce2f322118541b9901f218ce6e49177e5d25dd6c#body_text
is schema:about of	named entity 'DIAGNOSTIC' named entity 'CAUSE DISEASE' named entity 'APPLIED BIOSYSTEMS' named entity 'TOTAL' named entity 'DIARRHEA' »more»

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