About: Evaluation of Internal Reference Genes for Quantitative Expression Analysis by Real-Time PCR in Ovine Whole Blood

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Evaluation of Internal Reference Genes for Quantitative Expression Analysis by Real-Time PCR in Ovine Whole Blood
Creator	Ariello, Dario Bellino, Claudio Bertuzzi, Simone Campanella, Chiara Caramelli, Maria »more»
source	PMC
abstract	The use of reference genes is commonly accepted as the most reliable approach to normalize qRT-PCR and to reduce possible errors in the quantification of gene expression. The most suitable reference genes in sheep have been identified for a restricted range of tissues, but no specific data on whole blood are available. The aim of this study was to identify a set of reference genes for normalizing qRT-PCR from ovine whole blood. We designed 11 PCR assays for commonly employed reference genes belonging to various functional classes and then determined their expression stability in whole blood samples from control and disease-stressed sheep. SDHA and YWHAZ were considered the most suitable internal controls as they were stably expressed regardless of disease status according to both geNorm and NormFinder software; furthermore, geNorm indicated SDHA/HPRT, YWHAZ/GAPDH and SDHA/YWHAZ as the best reference gene combinations in control, disease-stressed and combined sheep groups, respectively. Our study provides a validated panel of optimal control genes which may be useful for the identification of genes differentially expressed by qRT-PCR in a readily accessible tissue, with potential for discovering new physiological and disease markers and as a tool to improve production traits (e.g., by identifying expression Quantitative Trait Loci). An additional outcome of the study is a set of intron-spanning primer sequences suitable for gene expression experiments employing SYBR Green chemistry on other ovine tissues and cells.
has issue date	2011-11-09(xsd:dateTime)
bibo:doi	10.3390/ijms12117732
bibo:pmid	22174628
has license	cc-by
sha1sum (hex)	cd21b40d05f66d9cc280d65928651c4552ad8fdd
schema:url	https://doi.org/10.3390/ijms12117732
resource representing a document's title	Evaluation of Internal Reference Genes for Quantitative Expression Analysis by Real-Time PCR in Ovine Whole Blood
has PubMed Central identifier	PMC3233434
has PubMed identifier	22174628
schema:publication	Int J Mol Sci
resource representing a document's body	covid:cd21b40d05f66d9cc280d65928651c4552ad8fdd#body_text
is schema:about of	named entity 'CONTROLS' named entity 'EXPRESSION ANALYSIS' named entity 'WHOLE BLOOD' named entity 'EMPLOYED' named entity 'BELONGING' »more»

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