About: Identification of neutralizing human monoclonal antibodies from Italian Covid-19 convalescent patients

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About: Identification of neutralizing human monoclonal antibodies from Italian Covid-19 convalescent patients Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Identification of neutralizing human monoclonal antibodies from Italian Covid-19 convalescent patients
Creator	Ippolito, Giuseppe Rappuoli, Rino Agrati, Chiara Castilletti, Concetta Fabbiani, Massimiliano »more»
source	BioRxiv
abstract	In the absence of approved drugs or vaccines, there is a pressing need to develop tools for therapy and prevention of Covid-19. Human monoclonal antibodies have very good probability of being safe and effective tools for therapy and prevention of SARS-CoV-2 infection and disease. Here we describe the screening of PBMCs from seven people who survived Covid-19 infection to isolate human monoclonal antibodies against SARS-CoV-2. Over 1,100 memory B cells were single-cell sorted using the stabilized prefusion form of the spike protein and incubated for two weeks to allow natural production of antibodies. Supernatants from each cell were tested by ELISA for spike protein binding, and positive antibodies were further tested for neutralization of spike binding to receptor(s) on Vero E6 cells and for virus neutralization in vitro. From the 1,167 memory B specific for SARS-CoV-2, we recovered 318 B lymphocytes expressing human monoclonals recognizing the spike protein and 74 of these were able to inhibit the binding of the spike protein to the receptor. Finally, 17 mAbs were able to neutralize the virus when assessed for neutralization in vitro. Lead candidates to progress into the drug development pipeline will be selected from the panel of neutralizing antibodies identified with the procedure described in this study. One Sentence Summary Neutralizing human monoclonal antibodies isolated from Covid-19 convalescent patients for therapeutic and prophylactic interventions.
has issue date	2020-05-09(xsd:dateTime)
bibo:doi	10.1101/2020.05.05.078154
has license	biorxiv
sha1sum (hex)	c9095de51802ad81812c8bf6c6594ac6cb3d1ab2
schema:url	https://doi.org/10.1101/2020.05.05.078154
resource representing a document's title	Identification of neutralizing human monoclonal antibodies from Italian Covid-19 convalescent patients
schema:publication	bioRxiv
resource representing a document's body	covid:c9095de51802ad81812c8bf6c6594ac6cb3d1ab2#body_text
is schema:about of	named entity 'inhibit' named entity 'human monoclonal antibodies' named entity 'panel' named entity 'develop' named entity 'human' »more»

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