About: Two mutations P/L and Y/C in SARS-CoV-2 helicase domain exist together and influence helicase RNA binding

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About: Two mutations P/L and Y/C in SARS-CoV-2 helicase domain exist together and influence helicase RNA binding Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Two mutations P/L and Y/C in SARS-CoV-2 helicase domain exist together and influence helicase RNA binding
Creator	Banerjee, Arup Begum, Feroza Ray, Upasana Tripathi, Prem
source	BioRxiv
abstract	RNA helicases play pivotal role in RNA replication by catalysing the unwinding of complex RNA duplex structures into single strands in ATP/NTP dependent manner. SARS coronavirus 2 (SARS-CoV-2) is a single stranded positive sense RNA virus belonging to the family Coronaviridae. The viral RNA encodes non structural protein Nsp13 or the viral helicase protein that helps the viral RNA dependent RNA polymerase (RdRp) to execute RNA replication by unwinding the RNA duplexes. In this study we identified a novel mutation at position 541of the helicase where the tyrosine (Y) got substituted with cytosine (C). We found that Y541C is a destabilizing mutation increasing the molecular flexibility and leading to decreased affinity of helicase binding with RNA. Earlier we had reported a mutation P504L in the helicase protein for which had not performed RNA binding study. Here we report that P504L mutation leads to increased affinity of helicase RNA interaction. So, both these mutations have opposite effects on RNA binding. Moreover, we found a significant fraction of isolate population where both P504L and Y541C mutations were co-existing.
has issue date	2020-05-15(xsd:dateTime)
bibo:doi	10.1101/2020.05.14.095224
has license	biorxiv
sha1sum (hex)	beb089fc4f251a5c95a21bcc7c13b26e21b9591b
schema:url	https://doi.org/10.1101/2020.05.14.095224
resource representing a document's title	Two mutations P/L and Y/C in SARS-CoV-2 helicase domain exist together and influence helicase RNA binding
schema:publication	bioRxiv
resource representing a document's body	covid:beb089fc4f251a5c95a21bcc7c13b26e21b9591b#body_text
is schema:about of	named entity 'viral' named entity 'binding' named entity 'domain' named entity 'ROLE' named entity 'PROTEIN ' »more»

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