About: Rapid production of clinical‐grade SARS‐CoV‐2 specific T cells

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Rapid production of clinical‐grade SARS‐CoV‐2 specific T cells
Creator	Leung, Wing Loh, Jiashen Tan, Guek, Jenny Low, Hong »more»
source	Medline; PMC
abstract	OBJECTIVES: To determine whether the frequencies of SARS‐CoV‐2‐specific T cells are sufficiently high in the blood of convalescent donors and whether it is technically feasible to manufacture clinical‐grade products overnight for T‐cell therapy and assessment of COVID‐19 immunity. METHODS: One unit of whole blood or leukapheresis was collected from each donor following standard blood bank practices. The leukocytes were stimulated using overlapping peptides of SARS‐CoV‐2, covering the immunodominant sequence domains of the S protein and the complete sequence of the N and M proteins. Thereafter, functionally reactive cells were enriched overnight using an automated device capturing IFNγ‐secreting cells. RESULTS: From 1 × 10(9) leukocytes, a median of 0.98 × 10(6) (range 0.56‐2.95) IFNγ + T cells were produced from each of the six donors, suggesting a high frequency of SARS‐CoV‐2 reactive T cells in their blood, even though only one donor had severe COVID‐19 requiring mechanical ventilation whereas the other five donors had minor symptoms. A median of 57% of the enriched T cells were IFNγ+ (range 20%‐74%), with preferential enrichment of CD56+ T cells and effector memory T cells. TCRVβ‐spectratyping confirmed distinctively tall oligoclonal peaks in final products. With just six donors, the probability that a recipient would share at least one HLA allele with one of the donors is >88% among Caucasian, >95% among Chinese, >97% among Malay, and >99% among Indian populations. CONCLUSIONS: High frequencies of rapid antigen‐reactive T cells were found in convalescent donors, regardless of severity of COVID‐19. The feasibility of clinical‐grade production of SARS‐CoV‐2‐specific T cells overnight for therapeutics and diagnostics is revealed.
has issue date	2020-07-31(xsd:dateTime)
bibo:doi	10.1002/acg2.101
bibo:pmid	32838213
has license	no-cc
sha1sum (hex)	b7f9f28c947dda23a940325fcb2e69f7d4bf7d35
schema:url	https://doi.org/10.1002/acg2.101
resource representing a document's title	Rapid production of clinical‐grade SARS‐CoV‐2 specific T cells
has PubMed Central identifier	PMC7404427
has PubMed identifier	32838213
schema:publication	Adv Cell Gene Ther
resource representing a document's body	covid:b7f9f28c947dda23a940325fcb2e69f7d4bf7d35#body_text
is schema:about of	named entity 'SARS-CoV-2' named entity 'SARS-CoV-2' named entity 'Rapid' named entity 'blood' named entity 'frequencies' »more»

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