About: Characterisation of porcine epidemic diarrhea virus isolates during the 2014

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About: Characterisation of porcine epidemic diarrhea virus isolates during the 2014–2015 outbreak in the Philippines Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Characterisation of porcine epidemic diarrhea virus isolates during the 2014–2015 outbreak in the Philippines
Creator	Balbin, Michelle Garcia, Gemerlyn Supnet, Jonathan Aquino, Arman Belotindos, P »more»
source	Medline; PMC
abstract	The viral agent of the porcine epidemic diarrhea (PED) was investigated during the reported 2014–2015 outbreaks in commercial farms in Central Luzon, Philippines. The study covered detection of PED virus (PEDV) in fecal and intestinal samples through reverse transcription PCR and sequence analysis of the nucleocapsid (N) gene. Results showed that 10 out of 34 fecal and intestinal samples examined were positive for PEDV. The partial nucleotide sequence of the N gene of the field samples showed 98–99% homologous to PEDV sequences registered in the GenBank. It was also noted that N gene sequences between field samples were 98% homologous. Interestingly, the partial sequences of the N genes of the field samples were genetically similar to the PEDV isolates from USA, China, Mexico, Canada and Japan. The phylogenetic tree analysis revealed that the Philippine samples clustered in group 2–1 of the PEDV, wherein the isolates of this group were responsible for the outbreaks in Asia and the USA. Analysis of the partial nucleotide and amino acid sequences revealed polymorphisms, deletions and insertions in the N-gene of the PEDV. Amino acid sequence alignment also showed deletions and insertion in the PEDV detected in the Philippines. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s13337-018-0470-4) contains supplementary material, which is available to authorized users.
has issue date	2018-06-28(xsd:dateTime)
bibo:doi	10.1007/s13337-018-0470-4
bibo:pmid	30159369
has license	bronze-oa
sha1sum (hex)	b6dd469a87f1c3f8bfb4a85f06e82829d1c7b332
schema:url	https://doi.org/10.1007/s13337-018-0470-4
resource representing a document's title	Characterisation of porcine epidemic diarrhea virus isolates during the 2014–2015 outbreak in the Philippines
has PubMed Central identifier	PMC6111962
has PubMed identifier	30159369
schema:publication	VirusDisease
resource representing a document's body	covid:b6dd469a87f1c3f8bfb4a85f06e82829d1c7b332#body_text
is schema:about of	named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'ISOLATES' named entity 'GenBank' named entity 'SEQUENCE ANALYSIS' named entity 'CENTRAL' »more»

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