About: Development of a PCR-and hybridization-based assay (PCR Adenovirus Consensus(®)) for the detection and the species identification of adenoviruses in respiratory specimens

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Development of a PCR-and hybridization-based assay (PCR Adenovirus Consensus(®)) for the detection and the species identification of adenoviruses in respiratory specimens Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Development of a PCR-and hybridization-based assay (PCR Adenovirus Consensus(®)) for the detection and the species identification of adenoviruses in respiratory specimens
Creator	Brouard, Jacques Corbet, Sandrine Duhamel, Jean-François Freymuth, François Gouarin, Stéphanie »more»
source	Elsevier; Medline; PMC
abstract	Background: Antigen detection assays and viral isolation techniques are routinely used to detect adenoviruses (Ad) associated with respiratory infections, and the value of the polymerase chain reaction (PCR) has recently been assessed. Objectives: This paper describes a PCR-hybridization-immunoenzymatic assay (PCR Adenovirus consensus(®)) used to detect Ad and identify Ad species in respiratory specimens. Results: On seven representative serotypes Ad 12, Ad 3, Ad 7, Ad 11, Ad 1, Ad 8, Ad 4, the mean genome equivalents per ml and the mean 50% infectious doses per ml were 10(6.3)and 10(4), respectively. Using 362 nasal aspirates from children, Ad were detected by immunofluorescence (IF) and culture in 97 cases (27%), by the PCR-Ad hexon method in 107 cases (29.5%) and by the PCR Adenovirus Consensus(®) method in 113 cases (31.2%); 13 samples were found positive by both PCR and negative by the IF and culture methods; five samples were only positive according to the PCR Adenovirus Consensus(®) method. The sensitivity, specificity, predictive positive value and predictive negative value of the PCR Adenovirus Consensus(®) method were 97.9%, 93.2%, 84%, 99.1%, respectively. The method identified the species (sp) from 91 positive amplicons: 1 Ad sp A, 44 Ad sp B, 42 Ad sp C, 3 Ad sp E, and 1 Ad sp F; 85 isolates were identified by IF or the neutralisation in culture, and 86 by a PCR-RE digestion method. The PCR Adenovirus Consensus(®) detected six positive samples that were negative according to the IF and culture methods, and it identified the precise species of nine IF-positive and culture-negative nasal aspirates. Conclusion: The PCR Adenovirus Consensus(®) technique is more efficient than the classical IF or culture techniques for the detection of Ad in respiratory samples. An internal control is included to validate the screening results, and specific probes are used to identify the Ad species.
has issue date	2004-07-07(xsd:dateTime)
bibo:doi	10.1016/j.jcv.2004.04.003
bibo:pmid	15364267
has license	no-cc
sha1sum (hex)	ab292a2d7ae194e60f7bf0408c09fa1fe1529faf
schema:url	https://doi.org/10.1016/j.jcv.2004.04.003
resource representing a document's title	Development of a PCR-and hybridization-based assay (PCR Adenovirus Consensus(®)) for the detection and the species identification of adenoviruses in respiratory specimens
has PubMed Central identifier	PMC7129058
has PubMed identifier	15364267
schema:publication	J Clin Virol
resource representing a document's body	covid:ab292a2d7ae194e60f7bf0408c09fa1fe1529faf#body_text
is schema:about of	named entity 'SPECIMENS' named entity 'RESULTS' named entity 'PAPER' named entity 'SCREENING' named entity 'CONTROL' »more»

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software