About: Synergistic inhibition of human cytomegalovirus replication by interferon-alpha/beta and interferon-gamma

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About: Synergistic inhibition of human cytomegalovirus replication by interferon-alpha/beta and interferon-gamma Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Synergistic inhibition of human cytomegalovirus replication by interferon-alpha/beta and interferon-gamma
Creator	Sainz, Bruno Garry, Robert Lamarca, Heather Morris, Cindy
source	Medline; PMC
abstract	BACKGROUND: Recent studies have shown that gamma interferon (IFN-γ) synergizes with the innate IFNs (IFN-α and IFN-β) to inhibit herpes simplex virus type 1 (HSV-1) replication in vitro. To determine whether this phenomenon is shared by other herpesviruses, we investigated the effects of IFNs on human cytomegalovirus (HCMV) replication. RESULTS: We have found that as with HSV-1, IFN-γ synergizes with the innate IFNs (IFN-α/β) to potently inhibit HCMV replication in vitro. While pre-treatment of human foreskin fibroblasts (HFFs) with IFN-α, IFN-β or IFN-γ alone inhibited HCMV plaque formation by ~30 to 40-fold, treatment with IFN-α and IFN-γ or IFN-β and IFN-γ inhibited HCMV plaque formation by 163- and 662-fold, respectively. The generation of isobole plots verified that the observed inhibition of HCMV plaque formation and replication in HFFs by IFN-α/β and IFN-γ was a synergistic interaction. Additionally, real-time PCR analyses of the HCMV immediate early (IE) genes (IE1 and IE2) revealed that IE mRNA expression was profoundly decreased in cells stimulated with IFN-α/β and IFN-γ (~5-11-fold) as compared to vehicle-treated cells. Furthermore, decreased IE mRNA expression was accompanied by a decrease in IE protein expression, as demonstrated by western blotting and immunofluorescence. CONCLUSION: These findings suggest that IFN-α/β and IFN-γ synergistically inhibit HCMV replication through a mechanism that may involve the regulation of IE gene expression. We hypothesize that IFN-γ produced by activated cells of the adaptive immune response may potentially synergize with endogenous type I IFNs to inhibit HCMV dissemination in vivo.
has issue date	2005-02-23(xsd:dateTime)
bibo:doi	10.1186/1743-422x-2-14
bibo:pmid	15727684
has license	cc-by
sha1sum (hex)	ab1ac4f7b9c57dad5e3d61f72e8e3d552059fa09
schema:url	https://doi.org/10.1186/1743-422x-2-14
resource representing a document's title	Synergistic inhibition of human cytomegalovirus replication by interferon-alpha/beta and interferon-gamma
has PubMed Central identifier	PMC554982
has PubMed identifier	15727684
schema:publication	Virol J
resource representing a document's body	covid:ab1ac4f7b9c57dad5e3d61f72e8e3d552059fa09#body_text
is schema:about of	named entity 'phenomenon' covid:arg/ab1ac4f7b9c57dad5e3d61f72e8e3d552059fa09 named entity 'virus' named entity 'HCMV' named entity 'replication' »more»

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