About: Development Optimization and Validation of RT-LAMP based COVID-19 Facility in Pakistan

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Development Optimization and Validation of RT-LAMP based COVID-19 Facility in Pakistan
Creator	Salman, Muhammad Ahad, Abdul Badar, Nazish Faraz, Muhammad Haq, Farhan »more»
source	BioRxiv
abstract	The pandemic SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2) has created a widespread panic across the globe especially in the developing countries like Pakistan. The lack of resources and technical staff are causing havoc challenges in the detection and prevention of this global outbreak. Therefore, a less expensive and massive screening of suspected individuals for COVID-19 is required. In this study, a user-friendly technique of reverse transcription-loop mediated isothermal amplification (RT-LAMP) was designed and validated to suggest a potential RT-qPCR alternate for rapid testing of COVID-19 suspected individuals. A total of 12 COVID-19 negative and 72 COVID-19 suspected individuals were analyzed. Both RT-qPCR and RT-LAMP assays were performed for all the individuals using open reading frame (ORF 1ab), nucleoprotein (N) and Spike (S) genes. All 12 specimens which were negative using RT-qPCR were also found negative using RT-LAMP assay. Overall 62 out of 72 positive samples (detected using RT-qPCR) were found COVID-19 positive using RT-LAMP assay. Interestingly all samples (45) having Ct values less than 30 showed 100% sensitivity. However, samples with weaker Ct values (i.e., => 35) showed 54% concordance, suggesting potential false negatives or false positives in RT-LAMP or RT-qPCR results, respectively. Overall comparative assessment showed that RT-LAMP assay showed strong sensitivity and specificity and can be used as an alternative strategy for rapid COVID-19 testing. Hence, based on fast processing time, minimal risk of specimens transfer and utilizing available resources, LAMP based detection of COVID-19 is strongly advocated especially for developing countries.
has issue date	2020-05-29(xsd:dateTime)
bibo:doi	10.1101/2020.05.29.124123
has license	biorxiv
sha1sum (hex)	a2bd62aa39c7ca10b49e31bb221e06f79c4e0837
schema:url	https://doi.org/10.1101/2020.05.29.124123
resource representing a document's title	Development Optimization and Validation of RT-LAMP based COVID-19 Facility in Pakistan
schema:publication	bioRxiv
resource representing a document's body	covid:a2bd62aa39c7ca10b49e31bb221e06f79c4e0837#body_text
is schema:about of	named entity 'massive' named entity 'genes' named entity 'detection' named entity 'individuals' named entity 'COVID-19' »more»

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