About: 2′-O methylation of the viral mRNA cap evades host restriction by IFIT family members

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About: 2′-O methylation of the viral mRNA cap evades host restriction by IFIT family members Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	2′-O methylation of the viral mRNA cap evades host restriction by IFIT family members
Creator	Thiel, Volker Gale, Michael Shi, Pei-Yong Daffis, Stephane Schriewer, Jill »more»
source	Medline; PMC
abstract	Cellular mRNA of higher eukaryotes and many viral RNA are methylated at the N-7 and 2′-O positions of the 5′ guanosine cap by specific nuclear and cytoplasmic methyltransferases (MTases), respectively. Whereas N-7 methylation is essential for RNA translation and stability 1, the function of 2′-O methylation has remained uncertain since its discovery 35 years ago 2-4. Here, we show that a West Nile virus (WNV) mutant (E218A) that lacks 2′-O MTase activity was attenuated in wild type primary cells and mice but was pathogenic in the absence of type I interferon (IFN) signaling. 2′-O methylation of viral RNA did not affect IFN induction in WNV-infected fibroblasts but instead modulated the antiviral effects of IFN-induced proteins with tetratricopeptide repeats (IFIT), which are interferon-stimulated genes (ISG) implicated in regulation of protein translation. Poxvirus and coronavirus mutants that lacked 2′-O MTase activity similarly showed enhanced sensitivity to the antiviral actions of IFN and specifically, IFIT proteins. Our results demonstrate that the 2′-O methylation of the 5′ cap of viral RNA functions to subvert innate host antiviral responses through escape of IFIT-mediated suppression, and suggest an evolutionary explanation for 2′-O methylation of cellular mRNA: to distinguish self from non-self RNA. Differential methylation of cytoplasmic RNA likely serves as a paradigm for pattern recognition and restriction of propagation of foreign viral RNA in host cells.
has issue date	2010-11-18(xsd:dateTime)
bibo:doi	10.1038/nature09489
bibo:pmid	21085181
has license	no-cc
sha1sum (hex)	a0cc0dc42e116ed81ab681809e67edadda9663c8
schema:url	https://doi.org/10.1038/nature09489
resource representing a document's title	2′-O methylation of the viral mRNA cap evades host restriction by IFIT family members
has PubMed Central identifier	PMC3058805
has PubMed identifier	21085181
schema:publication	Nature
resource representing a document's body	covid:a0cc0dc42e116ed81ab681809e67edadda9663c8#body_text
is schema:about of	named entity 'induction' named entity 'cytoplasmic' named entity 'full' named entity 'RNA' named entity 'viral' »more»

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