About: Structural Basis for Potent Neutralization of Betacoronaviruses by Single-domain Camelid Antibodies

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About: Structural Basis for Potent Neutralization of Betacoronaviruses by Single-domain Camelid Antibodies Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Structural Basis for Potent Neutralization of Betacoronaviruses by Single-domain Camelid Antibodies
Creator	Pöhlmann, Stefan Wrapp, Daniel Saelens, Xavier Schepens, Bert Roose, Kenny »more»
source	BioRxiv
abstract	The pathogenic Middle East respiratory syndrome coronavirus (MERS-CoV), severe acute respiratory syndrome coronavirus (SARS-CoV-1) and COVID-19 coronavirus (SARS-CoV-2) have all emerged into the human population with devastating consequences. These viruses make use of a large envelope protein called spike (S) to engage host cell receptors and catalyze membrane fusion. Because of the vital role that these S proteins play, they represent a vulnerable target for the development of therapeutics to combat these highly pathogenic coronaviruses. Here, we describe the isolation and characterization of single-domain antibodies (VHHs) from a llama immunized with prefusion-stabilized coronavirus spikes. These VHHs are capable of potently neutralizing MERS-CoV or SARS-CoV-1 S pseudotyped viruses. The crystal structures of these VHHs bound to their respective viral targets reveal two distinct epitopes, but both VHHs block receptor binding. We also show cross-reactivity between the SARS-CoV-1 S-directed VHH and SARS-CoV-2 S, and demonstrate that this cross-reactive VHH is capable of neutralizing SARS-CoV-2 S pseudotyped viruses as a bivalent human IgG Fc-fusion. These data provide a molecular basis for the neutralization of pathogenic betacoronaviruses by VHHs and suggest that these molecules may serve as useful therapeutics during coronavirus outbreaks.
has issue date	2020-03-28(xsd:dateTime)
bibo:doi	10.1101/2020.03.26.010165
has license	biorxiv
sha1sum (hex)	9ad2f17028295cf26f336f4d9fb32e4417af8c00
schema:url	https://doi.org/10.1101/2020.03.26.010165
resource representing a document's title	Structural Basis for Potent Neutralization of Betacoronaviruses by Single-domain Camelid Antibodies
schema:publication	bioRxiv
resource representing a document's body	covid:9ad2f17028295cf26f336f4d9fb32e4417af8c00#body_text
is schema:about of	named entity 'target' named entity 'pathogenic' named entity 'engage' named entity 'emerged' named entity 'large' »more»

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