About: In vitro assessment of the feline cell-mediated immune response against feline panleukopeniavirus, calicivirus and felid herpesvirus 1 using 5-bromo-2′-deoxyuridine labeling

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About: In vitro assessment of the feline cell-mediated immune response against feline panleukopeniavirus, calicivirus and felid herpesvirus 1 using 5-bromo-2′-deoxyuridine labeling Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	In vitro assessment of the feline cell-mediated immune response against feline panleukopeniavirus, calicivirus and felid herpesvirus 1 using 5-bromo-2′-deoxyuridine labeling
Creator	Dedeurwaerder, Annelike Nauwynck, Hans Dewerchin, Hannah Gleich, Sabine Desmarets, Lowiese »more»
source	Elsevier; Medline; PMC
abstract	In this study an in vitro assay was optimized to detect feline proliferating lymphocytes as an assessment for the cell-mediated immune response. For this purpose, 5-bromo-2′-deoxyuridine (BrdU) labeling was chosen because of its sensitivity and the possibility of further characterization of proliferating cells. The assay was optimized by selecting the best batch and concentration of fetal bovine serum, β-mercaptoethanol concentration, cell density, BrdU incubation time and antigen presenting cell type. Cats were vaccinated with the attenuated Nobivac vaccine Tricat and the peripheral blood lymphocyte proliferation responses were quantified upon in vitro restimulation with inactivated and infectious feline panleukopenia virus (FPV), feline calicivirus (FCV) and felid herpesvirus 1 (FeHV-1). Proliferation signals were detected with inactivated FeHV-1 in the CD8(+) but not in the CD8(−) T lymphocyte population, with inactivated FCV and FPV in both CD8(−) and CD8(+) T lymphocyte populations. Restimulation with infectious FCV caused significant proliferation in the CD8(−) T lymphocyte population only while infectious FPV and FeHV-1 seemed to suppress lymphocyte proliferation in both T cell populations. Additional IFN-γ quantification in the culture supernatant revealed a large correlation between the proliferation signals and IFN-γ production, indicating that BrdU labeling is a very reliable technique to assess and characterize feline lymphoproliferative responses to viral antigens in vitro.
has issue date	2012-04-15(xsd:dateTime)
bibo:doi	10.1016/j.vetimm.2012.03.004
bibo:pmid	22460172
has license	no-cc
sha1sum (hex)	9a4bad7a0eb4c15a9714774f969a3a3a7118630d
schema:url	https://doi.org/10.1016/j.vetimm.2012.03.004
resource representing a document's title	In vitro assessment of the feline cell-mediated immune response against feline panleukopeniavirus, calicivirus and felid herpesvirus 1 using 5-bromo-2′-deoxyuridine labeling
has PubMed Central identifier	PMC7112514
has PubMed identifier	22460172
schema:publication	Vet Immunol Immunopathol
resource representing a document's body	covid:9a4bad7a0eb4c15a9714774f969a3a3a7118630d#body_text
is schema:about of	named entity 'IN VITRO' named entity 'cell-mediated immune response' named entity 'feline' named entity 'culture' named entity 'FPV' »more»

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