About: Cell attenuated porcine epidemic diarrhea virus strain Zhejiang08 provides effective immune protection attributed to dendritic cell stimulation

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About: Cell attenuated porcine epidemic diarrhea virus strain Zhejiang08 provides effective immune protection attributed to dendritic cell stimulation Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Cell attenuated porcine epidemic diarrhea virus strain Zhejiang08 provides effective immune protection attributed to dendritic cell stimulation
Creator	Li, Yuchen Man, Kun Wang, Guihua Wang, Jialu Yang, Qian
source	Elsevier; Medline; PMC
abstract	Abstract Since 2010, the porcine epidemic diarrhea coronavirus (PEDV) has caused significant damage to the global pork industry. However, classical PEDV vaccine strains only provide limited protection against emerging strains. In this study, we successfully isolated and attenuated the PEDV epidemic strain Zhejiang08, which was characterized by good cell adaptation and high-titer production 48 h post infection in Vero E6 cells. The attenuated virus induced a high level of virus-specific neutralizing antibodies until 120 days after immunization in piglets and provided complete protection when challenged with an emerging virus strain on day 14 post immunization. Moreover, the capability to activate dendritic cells (DCs) of this isolate was identified. Higher expression levels of IL-12 and IFN-γ were recorded in DCs after treatment with Zhejiang08 for 24 h. Furthermore, genome sequencing and phylogenetic analysis revealed high homology between the main antigen epitopes of Zhejiang08 and PEDV pandemic isolates following 2011. Combining the glycosylation site prediction results and their distribution within the spatial structure of the S protein, led to the conclusion that the observed more effective host immune response of Zhejiang08 compared to CV777 was possibly associated with a lack of the potential glycosylation site in the 296 amino acids of the S protein. In summary, we illustrated that the attenuated virus represents a promising vaccine candidate.
has issue date	2017-12-15(xsd:dateTime)
bibo:doi	10.1016/j.vaccine.2017.10.052
bibo:pmid	29100707
has license	els-covid
sha1sum (hex)	99da3adecbf75588f2b231f28a660d32a8ed5829
schema:url	https://doi.org/10.1016/j.vaccine.2017.10.052
resource representing a document's title	Cell attenuated porcine epidemic diarrhea virus strain Zhejiang08 provides effective immune protection attributed to dendritic cell stimulation
has PubMed Central identifier	PMC7115645
has PubMed identifier	29100707
schema:publication	Vaccine
resource representing a document's body	covid:99da3adecbf75588f2b231f28a660d32a8ed5829#body_text
is schema:about of	named entity 'DCS' named entity 'VERO' named entity 'LIMITED' named entity 'FOLLOWING' named entity 'PROTECTION' »more»

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