About: C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system

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About: C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system
Creator	Canard, Bruno Decroly, Etienne Chiron, Stéphane Eaton, Heather Goncalves, Sergio »more»
source	PMC
abstract	Most eukaryotic expression systems make use of host-cell nuclear transcriptional and post-transcriptional machineries. Here, we present the first generation of the chimeric cytoplasmic capping-prone phage polymerase (C3P3-G1) expression system developed by biological engineering, which generates capped and polyadenylated transcripts in host-cell cytoplasm by means of two components. First, an artificial single-unit chimeric enzyme made by fusing an mRNA capping enzyme and a DNA-dependent RNA polymerase. Second, specific DNA templates designed to operate with the C3P3-G1 enzyme, which encode for the transcripts and their artificial polyadenylation. This system, which can potentially be adapted to any in cellulo or in vivo eukaryotic expression applications, was optimized for transient expression in mammalian cells. C3P3-G1 shows promising results for protein production in Chinese Hamster Ovary (CHO-K1) cells. This work also provides avenues for enhancing the performances for next generation C3P3 systems.
has issue date	2019-03-18(xsd:dateTime)
bibo:doi	10.1093/nar/gkz069
bibo:pmid	30726994
has license	cc-by-nc
sha1sum (hex)	95f7c797080ddb8d466a88443df62ea97f46d932
schema:url	https://doi.org/10.1093/nar/gkz069
resource representing a document's title	C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system
has PubMed Central identifier	PMC6412113
has PubMed identifier	30726994
schema:publication	Nucleic Acids Res
resource representing a document's body	covid:95f7c797080ddb8d466a88443df62ea97f46d932#body_text
is schema:about of	named entity 'machineries' named entity 'eukaryotic' named entity 'eukaryotic' covid:arg/95f7c797080ddb8d466a88443df62ea97f46d932 named entity 'expression' »more»

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