About: SAMD4 family members suppress human hepatitis B virus by directly binding to the Smaug recognition region of viral RNA

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About: SAMD4 family members suppress human hepatitis B virus by directly binding to the Smaug recognition region of viral RNA Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	SAMD4 family members suppress human hepatitis B virus by directly binding to the Smaug recognition region of viral RNA
Creator	Xia, Ningshao Li, Xiaoling Liu, Yifei Wu, Jianfeng Yuan, Quan »more»
source	PMC
abstract	HBV infection initiates hepatitis B and promotes liver cirrhosis and hepatocellular carcinoma. IFN-α is commonly used in hepatitis B therapy, but how it inhibits HBV is not fully understood. We screened 285 human interferon-stimulated genes (ISGs) for anti-HBV activity using a cell-based assay, which revealed several anti-HBV ISGs. Among these ISGs, SAMD4A was the strongest suppressor of HBV replication. We found the binding site of SAMD4A in HBV RNA, which was a previously unidentified Smaug recognition region (SRE) sequence conserved in HBV variants. SAMD4A binds to the SRE site in viral RNA to trigger its degradation. The SAM domain in SAMD4A is critical for RNA binding and the C-terminal domain of SAMD4A is required for SAMD4A anti-HBV function. Human SAMD4B is a homolog of human SAMD4A but is not an ISG, and the murine genome encodes SAMD4. All these SAMD4 proteins suppressed HBV replication when overexpressed in vitro and in vivo. We also showed that knocking out the Samd4 gene in hepatocytes led to a higher level of HBV replication in mice and AAV-delivered SAMD4A expression reduced the virus titer in HBV-producing transgenic mice. In addition, a database analysis revealed a negative correlation between the levels of SAMD4A/B and HBV in patients. Our data suggest that SAMD4A is an important anti-HBV ISG for use in IFN therapy of hepatitis B and that the levels of SAMD4A/B expression are related to HBV sensitivity in humans.
has issue date	2020-04-27(xsd:dateTime)
bibo:doi	10.1038/s41423-020-0431-x
bibo:pmid	32341522
has license	no-cc
sha1sum (hex)	93fc17be871494623d0b5cbd8b53125748e6ad42
schema:url	https://doi.org/10.1038/s41423-020-0431-x
resource representing a document's title	SAMD4 family members suppress human hepatitis B virus by directly binding to the Smaug recognition region of viral RNA
has PubMed Central identifier	PMC7223975
has PubMed identifier	32341522
schema:publication	Cell Mol Immunol
resource representing a document's body	covid:93fc17be871494623d0b5cbd8b53125748e6ad42#body_text
is schema:about of	named entity 'RNA' named entity 'reduced' named entity 'SAM' named entity 'SRE' named entity 'Smaug' »more»

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