About: Evaluation of Allplex Respiratory Panel 1/2/3 Multiplex Real-Time PCR Assays for the Detection of Respiratory Viruses with Influenza A Virus subtyping

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Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Evaluation of Allplex Respiratory Panel 1/2/3 Multiplex Real-Time PCR Assays for the Detection of Respiratory Viruses with Influenza A Virus subtyping
Creator	Lee, Jaehyeon Cho, Yong Lee, Hye Choi, Sam Kim, Dal
topic	covid:8f810a6f1464d6871d6184e04cf3fb1417983abc#this
source	Medline; PMC
abstract	The Allplex Respiratory Panel 1/2/3 (All16) is a multiplex PCR assay for detecting 16 respiratory viruses with influenza A virus (FluA) subtyping, and the first clinical assay based on multiple detection temperatures. We compared the results between All16 and Anyplex II RV16 (Any16) in 426 clinical samples. Samples showing discrepancies between the two tests were further tested using monoplex PCR. FluA subtyping based on the hemagglutinin type results of All16, which yielded H1, H3, and non-H1/H3, was compared with the results of the BioFire FilmArray respiratory panel. The positive and negative percent agreements and kappa value for each virus between All16 and Any16 ranged from 54.5-100.0%, 84.7-100.0%, and 0.57-1.00, respectively. FluA subtype results from All16 for 26 samples were consistent with those from FilmArray. Good agreement was observed between the two methods, except when analyzing human enterovirus (kappa value 0.70), and the All16 showed reliable FluA subtyping results. For parainfluenza virus 3, the All16 was more sensitive than Any16. When testing 28 samples simultaneously, the mean test time and hands-on time were 4.3 and 0.5 hours, respectively in All16. In conclusion, All16 showed reliable performance, but further studies are needed regarding human enterovirus analysis.
has issue date	2017-10-23(xsd:dateTime)
bibo:doi	10.3343/alm.2018.38.1.46
bibo:pmid	29071818
has license	cc-by-nc
sha1sum (hex)	8f810a6f1464d6871d6184e04cf3fb1417983abc
schema:url	https://doi.org/10.3343/alm.2018.38.1.46
resource representing a document's title	Evaluation of Allplex Respiratory Panel 1/2/3 Multiplex Real-Time PCR Assays for the Detection of Respiratory Viruses with Influenza A Virus subtyping
has PubMed Central identifier	PMC5700146
has PubMed identifier	29071818
schema:publication	Ann Lab Med
resource representing a document's body	covid:8f810a6f1464d6871d6184e04cf3fb1417983abc#body_text
is http://vocab.deri.ie/void#inDataset of	https://covidontheweb.inria.fr:4443/about/id/http/ns.inria.fr/covid19/8f810a6f1464d6871d6184e04cf3fb1417983abc
is schema:about of	named entity 'COMMUNICATION' named entity 'BUT' named entity 'ATTRIBUTION' named entity 'NEEDED' named entity 'RESULTS' »more»

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