About: Inhibitory effect of flavonoids against NS2B-NS3 protease of ZIKA virus and their structure activity relationship

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About: Inhibitory effect of flavonoids against NS2B-NS3 protease of ZIKA virus and their structure activity relationship Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Inhibitory effect of flavonoids against NS2B-NS3 protease of ZIKA virus and their structure activity relationship
Creator	Kim, Nguyen, Park, Kim, Jun-Seong Lim, Hee-Jung »more»
source	Medline; PMC
abstract	OBJECTIVES: To determine the inhibitory activities of flavonoids against NS2B-NS3 protease of ZIKA virus (ZIKV NS2B-NS3(pro)) expressed in Escherichia coli BL21 (DE3) and their structure activity relationship. RESULTS: ZIKV NS2B-NS3(pro) was expressed in E. coli BL21(DE3) as a 35 kDa protein. It had a K (m) of 26 µM with the fluorogenic peptide Dabcyl-KTSAVLQSGFRKME-Edan. The purified ZIKV NS2B-NS3(pro) was used for inhibition and kinetic assays to determine the activities of 22 polyphenol compounds. These polyphenol compounds at 100 µM inhibited the activity of ZIKV NS2B-NS3(pro) by 6.2–88%. Seven polyphenol compounds had IC(50) ranging from 22 ± 0.2 to 112 ± 5.5 µM. Myricetin showed a mixed type inhibitory pattern against ZIKV NS2B-NS3(pro) protease. Its IC(50) value was 22 ± 0.2 µM with a K (i) value of 8.9 ± 1.9 µM. CONCLUSION: The chemical structure of a polyphenol compound and its inhibitory activity against ZIKV NS2B-NS3(pro) can be explored to develop highly selective inhibitors against ZIKV NS2B-NS3(pro). ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10529-016-2261-6) contains supplementary material, which is available to authorized users.
has issue date	2016-11-24(xsd:dateTime)
bibo:doi	10.1007/s10529-016-2261-6
bibo:pmid	27885509
has license	no-cc
sha1sum (hex)	85a219a54de089e3d0923da0959cfc9d2c80fdd6
schema:url	https://doi.org/10.1007/s10529-016-2261-6
resource representing a document's title	Inhibitory effect of flavonoids against NS2B-NS3 protease of ZIKA virus and their structure activity relationship
has PubMed Central identifier	PMC7088272
has PubMed identifier	27885509
schema:publication	Biotechnol Lett
resource representing a document's body	covid:85a219a54de089e3d0923da0959cfc9d2c80fdd6#body_text
is schema:about of	named entity 'polyphenol' named entity 'kinetic' named entity 'polyphenol' named entity 'pro' named entity 'structure activity relationship' »more»

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