About: The nonstructural protein 8 (nsp8) of the SARS coronavirus interacts with its ORF6 accessory protein

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	The nonstructural protein 8 (nsp8) of the SARS coronavirus interacts with its ORF6 accessory protein
Creator	Tan, Yee-Joo Kumar, Purnima Druce, Julian Birch, Chris Catton, Mike »more»
source	Elsevier; Medline; PMC
abstract	Abstract Severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) caused a severe outbreak in several regions of the world in 2003. The SARS-CoV genome is predicted to contain 14 functional open reading frames (ORFs). The first ORF (1a and 1b) encodes a large polyprotein that is cleaved into nonstructural proteins (nsp). The other ORFs encode for four structural proteins (spike, membrane, nucleocapsid and envelope) as well as eight SARS-CoV-specific accessory proteins (3a, 3b, 6, 7a, 7b, 8a, 8b and 9b). In this report we have cloned the predicted nsp8 gene and the ORF6 gene of the SARS-CoV and studied their abilities to interact with each other. We expressed the two proteins as fusion proteins in the yeast two-hybrid system to demonstrate protein–protein interactions and tested the same using a yeast genetic cross. Further the strength of the interaction was measured by challenging growth of the positive interaction clones on increasing gradients of 2-amino trizole. The interaction was then verified by expressing both proteins separately in-vitro in a coupled-transcription translation system and by coimmunoprecipitation in mammalian cells. Finally, colocalization experiments were performed in SARS-CoV infected Vero E6 mammalian cells to confirm the nsp8–ORF6 interaction. To the best of our knowledge, this is the first report of the interaction between a SARS-CoV accessory protein and nsp8 and our findings suggest that ORF6 protein may play a role in virus replication.
has issue date	2007-09-30(xsd:dateTime)
bibo:doi	10.1016/j.virol.2007.04.029
bibo:pmid	17532020
has license	els-covid
sha1sum (hex)	82f87f9e25518fb169113d7e891a5f8106e82133
schema:url	https://doi.org/10.1016/j.virol.2007.04.029
resource representing a document's title	The nonstructural protein 8 (nsp8) of the SARS coronavirus interacts with its ORF6 accessory protein
has PubMed Central identifier	PMC7103355
has PubMed identifier	17532020
schema:publication	Virology
resource representing a document's body	covid:82f87f9e25518fb169113d7e891a5f8106e82133#body_text
is schema:about of	named entity 'nucleocapsid' named entity 'predicted' named entity 'encode' named entity 'outbreak' named entity 'SARS CORONAVIRUS' »more»

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