About: Multilocus typing of Cryptosporidium spp. in young calves with diarrhea in Korea

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Multilocus typing of Cryptosporidium spp. in young calves with diarrhea in Korea
Creator	Oh, Sang-Ik Kim, Ha-Young Lee, Yu-Ran Chae, Myeongju Goo, Youn-Kyoung »more»
source	Elsevier; Medline; PMC
abstract	Abstract We assessed the prevalence and performed molecular analysis of Cryptosporidium spp. in diarrheal feces of calves in Korea. Diarrheal fecal samples were collected from 951 young calves (<3months) on 425 farms. Cryptosporidium prevalence was assessed by PCR and ELISA, and molecular characterization was performed by targeting the 18S rRNA, heat-shock protein 70 (hsp70), and glycoprotein 60 (gp60) genes. Data were analyzed according to the sex, type of cattle, region, season, and type of diarrhea. PCR analysis revealed Cryptosporidium spp. in 9.9% (94/951) of diarrheal fecal samples. C. parvum and C. bovis/ryanae were present in 6.1% (58/951) and 4.1% (39/951) of diarrheal fecal samples, respectively. In addition, ELISA showed positive results for C. parvum in 9.7% (92/951) samples. Statistical analysis of the PCR and ELISA results revealed a lower prevalence of C. parvum in the hemorrhagic diarrheal samples (P <0.05). For C. bovis/ryanae, seasonality and high prevalence in hemorrhagic diarrhea were observed (P <0.05). Of the 951 samples tested for C. parvum, 903 samples showed agreement with a κ value of 0.65, indicating good agreement between the two tests. Although C. bovis and C. ryanae share highly similar 18S rRNA sequences, PCR based on hsp70 successfully distinguished C. bovis from C. ryanae. Sequence analysis of gp60 revealed that C. parvum belonged to the IIa families and was further subtyped as IIaA18G3R1 and IIaA16G3R1, which have not been previously reported in Asia. These findings indicate that Cryptosporidium spp. play an important role in diarrhea in young calves in Korea. Considering the zoonotic significance of C. parvum IIa subtype and dense rearing system of cattle in Korea, prevention and continuous monitoring of Cryptosporidium are required.
has issue date	2016-10-15(xsd:dateTime)
bibo:doi	10.1016/j.vetpar.2016.09.019
bibo:pmid	27809984
has license	els-covid
sha1sum (hex)	8143c624d28df7b1014cbfc211109f0a0fbb5688
schema:url	https://doi.org/10.1016/j.vetpar.2016.09.019
resource representing a document's title	Multilocus typing of Cryptosporidium spp. in young calves with diarrhea in Korea
has PubMed Central identifier	PMC7185498
has PubMed identifier	27809984
schema:publication	Veterinary Parasitology
resource representing a document's body	covid:8143c624d28df7b1014cbfc211109f0a0fbb5688#body_text
is schema:about of	named entity 'C. parvum' named entity 'cattle' named entity 'fecal' named entity 'Korea' named entity 'For' »more»

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