About: The expression of SARS–CoV M gene in P. Pastoris and the diagnostic utility of the expression product

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About: The expression of SARS–CoV M gene in P. Pastoris and the diagnostic utility of the expression product Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	The expression of SARS–CoV M gene in P. Pastoris and the diagnostic utility of the expression product
Creator	Bartlam, Mark Rao, Zihe Cai, Xuepeng Liu, Xiangtao Han, Xueqing »more»
source	Elsevier; Medline; PMC
abstract	High-level protein expression is an important means of obtaining large amounts of viral proteins to investigate further their biological properties. To express the membrane (M) protein of SARS–CoV at high-level in vitro, the M gene fragment was amplified and cloned it into the Pichia Pastoris expression vector pPICZαA. SDS–PAGE and Western blotting analysis of the induced products of recombinant yeast transformant indicated that successful high-level expression of M protein was achieved, and that the expression product was similar antigenically to the natural protein. Purified recombinant M protein was used subsequently as an ELISA antigen for detection of eight serum samples screened previously by whole virus ELISA and immunofluorescence assay, and consistent results were obtained. These findings suggest that the recombinant M protein may be useful as a diagnostic reagent.
has issue date	2004-12-01(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2004.08.015
bibo:pmid	15488627
has license	no-cc
sha1sum (hex)	7d7da3a23085ac998f2681c9af584fa9b11505b8
schema:url	https://doi.org/10.1016/j.jviromet.2004.08.015
resource representing a document's title	The expression of SARS–CoV M gene in P. Pastoris and the diagnostic utility of the expression product
has PubMed Central identifier	PMC7112794
has PubMed identifier	15488627
schema:publication	J Virol Methods
resource representing a document's body	covid:7d7da3a23085ac998f2681c9af584fa9b11505b8#body_text
is schema:about of	named entity 'gene' named entity 'Pichia Pastoris' named entity 'proteins' named entity 'detection' named entity 'PURIFIED' »more»

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