About: A pilot study on primary cultures of human respiratory tract epithelial cells to predict patients’ responses to H7N9 infection

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About: A pilot study on primary cultures of human respiratory tract epithelial cells to predict patients’ responses to H7N9 infection Goto Sponge NotDistinct Permalink

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	A pilot study on primary cultures of human respiratory tract epithelial cells to predict patients’ responses to H7N9 infection
Creator	Huang, Chung-Guei Chen, Min-Chi Chen, Tse-Ching Horng, Jim-Tong Hsiao, Mei-Jen »more»
source	PMC
abstract	Avian influenza A(H7N9) virus infections frequently lead to acute respiratory distress syndrome and death in humans. We aimed to investigate whether primary cultures of human respiratory tract epithelial cells are helpful to understand H7N9 virus pathogenesis and tissue tropism, and to evaluate how patient-related characteristics can affect the host's response to infection. Normal human bronchial epithelial cells (isolated from two different donors) and primary epithelial cells (harvested from 27 patients undergoing airway surgery) were experimentally infected with H7N9 and/or H1N1pdm for 72 h. After virus infection, the culture media were collected for viral RNA quantitation and cytokine detection. Both H7N9 and H1N1pdm viruses replicated and induced a cytokine response differently for each donor in the normal human bronchial epithelial model. H7N9 replicated equivalently in epithelial cells harvested from the inferior turbinate and paranasal sinus, and those from the larynx and bronchus, at 72 h post-infection. Viral RNA quantity at 72 h was significantly higher in patients aged 21–64 years than in patients aged ≥ 65 years; however, no effects of sex, medical comorbidities, and obesity were noted. H7N9-infected cultured cells released multiple cytokines within 72 h. Levels of interleukin-1β, interleukin-6, interleukin-8, interferon-γ, and tumor necrosis factor-α were associated differently with patient-related characteristics (such as age, sex, obesity, and medical comorbidities). In the era of precision medicine, these findings illustrate the potential utility of this primary culture approach to predict a host's response to H7N9 infection or to future infection by newly emerging viral infections, and to dissect viral pathogenesis.
has issue date	2018-02-20(xsd:dateTime)
bibo:doi	10.18632/oncotarget.24537
bibo:pmid	29581859
has license	cc-by
sha1sum (hex)	7a488aaf9773826634d7018c0da282421523ba6c
schema:url	https://doi.org/10.18632/oncotarget.24537
resource representing a document's title	A pilot study on primary cultures of human respiratory tract epithelial cells to predict patients’ responses to H7N9 infection
has PubMed Central identifier	PMC5865685
has PubMed identifier	29581859
schema:publication	Oncotarget
resource representing a document's body	covid:7a488aaf9773826634d7018c0da282421523ba6c#body_text
is schema:about of	named entity 'distributed' named entity 'Copyright' named entity 'Creative Commons Attribution License' named entity 'terms' named entity 'USE' »more»

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