About: Bypass of DNA Interstrand crosslinks by a Rev1-DNA polymerase ζ complex

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About: Bypass of DNA Interstrand crosslinks by a Rev1-DNA polymerase ζ complex Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Bypass of DNA Interstrand crosslinks by a Rev1-DNA polymerase ζ complex
Creator	Burgers, Peter Bezalel-Buch, Rachel Cheun, Young Roy, Upasana Schärer, Orlando
source	BioRxiv
abstract	DNA polymerase ζ (Pol ζ) and Rev1 are essential for the repair of DNA interstrand crosslink (ICL) damage. We have used yeast DNA polymerases η, ζ, and Rev1 to study translesion synthesis (TLS) past a nitrogen mustard-based ICL with an 8-atom linker between the crosslinked bases. The Rev1-Pol ζ complex was most efficient in complete bypass synthesis, by 2-3 fold, compared to Pol ζ alone or Pol η. Rev1 protein, but not its catalytic activity, was required for efficient TLS. A dCMP residue was faithfully inserted across the ICL-G by Pol η, Pol ζ, and Rev1-Pol ζ. Rev1-Pol ζ, and particularly Pol ζ alone showed a tendency to stall before the ICL, whereas Pol η stalled just after insertion across the ICL. The stalling of Pol η directly past the ICL is attributed to its autoinhibitory activity, caused by elongation of the short ICL-unhooked oligonucleotide (a six-mer in our study) by Pol η providing a barrier to further elongation of the correct primer. No stalling by Rev1-Pol ζ directly past the ICL was observed, suggesting that the proposed function of Pol ζ as an extender DNA polymerase is also required for ICL repair.
has issue date	2020-05-10(xsd:dateTime)
bibo:doi	10.1101/2020.05.09.085902
has license	biorxiv
sha1sum (hex)	79d10dca01349190044f68c7f2a26e00545bf788
schema:url	https://doi.org/10.1101/2020.05.09.085902
resource representing a document's title	Bypass of DNA Interstrand crosslinks by a Rev1-DNA polymerase ζ complex
schema:publication	bioRxiv
resource representing a document's body	covid:79d10dca01349190044f68c7f2a26e00545bf788#body_text
is schema:about of	named entity 'Pol' named entity 'observed' named entity 'Pol' named entity 'polymerase' named entity 'yeast' »more»

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