About: SARS-CoV2 quantification using RT-dPCR: a faster and safer alternative to assist viral genomic copies assessment using RT-qPCR

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About: SARS-CoV2 quantification using RT-dPCR: a faster and safer alternative to assist viral genomic copies assessment using RT-qPCR Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	SARS-CoV2 quantification using RT-dPCR: a faster and safer alternative to assist viral genomic copies assessment using RT-qPCR
Creator	Karolina, Ana Rodrigues De Almeida, Paula Demoliner¹, Meriane Eisen¹, Antunes Fleck¹, Juliane »more»
source	BioRxiv
abstract	In this study, serial dilutions of SARS-CoV 2 RNA extract were tested using RT-dPCR using three different primer-probe assays aiming SARS-CoV 2 nucleocapsid coding region. Narrower confidence intervals, indicating high quantification precision were obtained in 100 and 1000-fold serial dilution and RT-dPCR results were equivalent between different assays in the same dilution. High accuracy of this test allowed conclusions regarding the ability of this technique to evaluate precisely the amount of genomic copies present in a sample. We believe that this fast and safe method can assist other researchers in titration of SARS-CoV2 controls used in RT-qPCR without the need of virus isolation.
has issue date	2020-05-01(xsd:dateTime)
bibo:doi	10.1101/2020.05.01.072728
has license	biorxiv
sha1sum (hex)	7364613efdf21241a54ae67572eb0a2e39c76683
schema:url	https://doi.org/10.1101/2020.05.01.072728
resource representing a document's title	SARS-CoV2 quantification using RT-dPCR: a faster and safer alternative to assist viral genomic copies assessment using RT-qPCR
schema:publication	bioRxiv
resource representing a document's body	covid:7364613efdf21241a54ae67572eb0a2e39c76683#body_text
is schema:about of	named entity 'nucleocapsid' named entity 'researchers' named entity 'GENOMIC' named entity 'SERIAL' named entity '1000' »more»

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