About: Detection of equine arteritis virus (EAV) by polymerase chain reaction (PCR) and differentiation of EAV strains by restriction enzyme analysis of PCR products

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About: Detection of equine arteritis virus (EAV) by polymerase chain reaction (PCR) and differentiation of EAV strains by restriction enzyme analysis of PCR products Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Detection of equine arteritis virus (EAV) by polymerase chain reaction (PCR) and differentiation of EAV strains by restriction enzyme analysis of PCR products
Creator	Kondo, T Wada, R Fuktmaga, Y Kamada, M Sekiguchi, K »more»
source	PMC
abstract	A polymerase chain reaction (PCR) based assay capable of detecting and differentiating seven strains of equine arteritis virus (EAV) from around the world was developed. The primers for the PCR were chosen from the ORF 6 gene encoding the unglycosylated membrane protein (M). Viral RNA from cell culture fluids infected with each of the seven EAV strains and RNA from the live vaccine, Arvac, was detected by PCR using four sets of primers. The sensitivity of detection was increased from 100 to 1000 times by performing nested PCR enabling the detection of RNA at a level of 0.5–5 PFU. Differentiation among the virus strains and the live vaccine was achieved by cutting the PCR-amplified products from three sets of primers with six restriction endonucleases. Using this procedure it was possible to distinguish among the seven EAV strains used.
has issue date	1995-01-01(xsd:dateTime)
bibo:doi	10.1007/bf01322675
bibo:pmid	7661700
has license	no-cc
sha1sum (hex)	72fc688d0a9aa57f9ca59d40f1eaa4ea89d68285
schema:url	https://doi.org/10.1007/bf01322675
resource representing a document's title	Detection of equine arteritis virus (EAV) by polymerase chain reaction (PCR) and differentiation of EAV strains by restriction enzyme analysis of PCR products
has PubMed Central identifier	PMC7087138
has PubMed identifier	7661700
schema:publication	Arch Virol
resource representing a document's body	covid:72fc688d0a9aa57f9ca59d40f1eaa4ea89d68285#body_text
is schema:about of	named entity 'EAV' named entity 'chosen' named entity 'PCR' named entity 'Differentiation' named entity 'MEMBRANE PROTEIN' »more»

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