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About:
Rapid Detection of Novel Coronavirus (COVID-19) by Reverse Transcription-Loop-Mediated Isothermal Amplification
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An Entity of Type :
schema:ScholarlyArticle
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covidontheweb.inria.fr
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Academic Article
research paper
schema:ScholarlyArticle
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type
Academic Article
research paper
schema:ScholarlyArticle
isDefinedBy
Covid-on-the-Web dataset
title
Rapid Detection of Novel Coronavirus (COVID-19) by Reverse Transcription-Loop-Mediated Isothermal Amplification
Creator
Bartolone, Sarah
Chancellor, Michael
Lamb, Laura
Ward, Elijah
source
MedRxiv
abstract
Novel Corona virus (COVID-19 or 2019-nCoV) is an emerging global health concern that requires a rapid diagnostic test. Quantitative reverse transcription PCR (qRT-PCR) is currently the standard for COVID-19 detection; however, Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) may allow for faster and cheaper field based testing at point-of-risk. The objective of this study was to develop a rapid screening diagnostic test that could be completed in under 30 minutes. Simulated patient samples were generated by spiking serum, urine, saliva, oropharyngeal swabs, and nasopharyngeal swabs with a portion of the COVID-19 nucleic sequence. The samples were tested using RT-LAMP as well as by conventional qRT-PCR. Specificity of the RT-LAMP was evaluated by also testing against other related coronaviruses. RT-LAMP specifically detected COVID-19 in simulated patient samples. This test was performed in under 30 minutes. This approach could be used for monitoring of exposed individuals or potentially aid with screening efforts in the field and potential ports of entry.
has issue date
2020-02-24
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bibo:doi
10.1101/2020.02.19.20025155
has license
medrxiv
sha1sum (hex)
712f1a74a5083d690832eed5fd9ff16a04434d8d
schema:url
https://doi.org/10.1101/2020.02.19.20025155
resource representing a document's title
Rapid Detection of Novel Coronavirus (COVID-19) by Reverse Transcription-Loop-Mediated Isothermal Amplification
resource representing a document's body
covid:712f1a74a5083d690832eed5fd9ff16a04434d8d#body_text
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schema:about
of
named entity 'The samples'
named entity 'reverse transcription PCR'
named entity 'Corona'
named entity 'testing'
named entity 'diagnostic test'
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