About: SARS Coronavirus Spike Protein Expression in HL-CZ Human Promonocytic Cells: Monoclonal Antibody and Cellular Transcriptomic Analyses

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About: SARS Coronavirus Spike Protein Expression in HL-CZ Human Promonocytic Cells: Monoclonal Antibody and Cellular Transcriptomic Analyses Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	SARS Coronavirus Spike Protein Expression in HL-CZ Human Promonocytic Cells: Monoclonal Antibody and Cellular Transcriptomic Analyses
Creator	Chow, Vincent Chow, V Lal, S Goudsmit, J Narasaraju, T »more»
source	PMC
abstract	The SARS coronavirus (CoV) spike protein is a target of intensive research, as it is a major virulence factor. Transfection of SARS-CoV spike into Vero E6, HEK293T and HL-CZ cells leads to strong expression of the glycosylated spike protein, as shown by Western blot analyses and immunofluorescent imaging using spike-specific human monoclonal antibodies, indicating the potential utility of these antigens and antibodies as diagnostic reagents. Furthermore, we employed cDNA microarray analysis to probe the changes in host gene transcription attributed to transfection of a codon-optimized spike construct into the HL-CZ cell line of monocyte lineage that is linked to immunological responses. A diverse representation of 100 genes displayed altered transcriptional patterns in response to SARS-CoV spike expression, with 61 upregulated and 39 downregulated genes. Genes involved in intracellular trafficking, signaling, modulation or transcription were generally upregulated. In contrast, genes involved in cell metabolism and cytoskeleton formation were notably downregulated. The transcripts of other functional categories exhibited varied responses to SARS-CoV spike transfection. Collectively, our analyses elucidate numerous and complex transcriptomic events that occur in response to spike protein expression and that contribute towards SARS-CoV pathogenesis.
has issue date	2009-07-22(xsd:dateTime)
bibo:doi	10.1007/978-3-642-03683-5_18
has license	no-cc
sha1sum (hex)	6e0a9b451aaf21661cf05f2c6f01cda9c8585de3
schema:url	https://doi.org/10.1007/978-3-642-03683-5_18
resource representing a document's title	SARS Coronavirus Spike Protein Expression in HL-CZ Human Promonocytic Cells: Monoclonal Antibody and Cellular Transcriptomic Analyses
has PubMed Central identifier	PMC7176169
schema:publication	Molecular Biology of the SARS-Coronavirus
resource representing a document's body	covid:6e0a9b451aaf21661cf05f2c6f01cda9c8585de3#body_text
is schema:about of	named entity 'formation' named entity 'SARS-CoV' named entity 'SARS-CoV' named entity 'probe' named entity 'monocyte' »more»

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