About: Cloning and Sequence Analysis of N Gene of Transmissible Gastroenteritis Virus HYM-09 Isolated from Dog in China

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About: Cloning and Sequence Analysis of N Gene of Transmissible Gastroenteritis Virus HYM-09 Isolated from Dog in China Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Cloning and Sequence Analysis of N Gene of Transmissible Gastroenteritis Virus HYM-09 Isolated from Dog in China
Creator	Chaolai, Man Xiaolong, Yu
source	Medline; PMC
abstract	Transmissible gastroenteritis virus (TGEV) is the etiological agent of TGE, and dogs are potential carriers of TGEV. In this study, genomic RNA were extracted from TGEV designated HYM-09 isolated from dog naturally infected with TGEV. The nucleocapsid (N) gene of HYM-09 was amplified by RT-PCR and cloned into pMD18-T vector. The N gene cDNA was sequenced and encompassed an open reading frame of 1,149 nucleotides, encoding a 382-amino acids protein. Sequence analyses of the N genes were performed, including homologous comparison, phylogenetic tree analysis and residue substitution analysis. The results showed that there existed some unique mutations in the HYM-09 isolate N gene, but HYM-09 N gene shared over 96 % homologous identities compared with 12 TGEV reference strains derived from other regions or countries respectively. The phylogenetic tree analysis revealed that the HYM-09 branched into the most strains group. This study shows that the nucleotide sequence analysis can form a base or further study on the mutation trend of non-porcine TGEV.
has issue date	2012-08-14(xsd:dateTime)
bibo:doi	10.1007/s13337-012-0072-5
bibo:pmid	24293826
has license	green-oa
sha1sum (hex)	6d43b16413a87651f3c1cf9a7c9bd7f6d8cfb20e
schema:url	https://doi.org/10.1007/s13337-012-0072-5
resource representing a document's title	Cloning and Sequence Analysis of N Gene of Transmissible Gastroenteritis Virus HYM-09 Isolated from Dog in China
has PubMed Central identifier	PMC3550793
has PubMed identifier	24293826
schema:publication	Indian Journal of Virology
resource representing a document's body	covid:6d43b16413a87651f3c1cf9a7c9bd7f6d8cfb20e#body_text
is schema:about of	named entity 'potential' named entity 'RT-PCR' named entity 'Gastroenteritis' named entity 'Cloning' named entity 'China' »more»

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