About: High-surety isothermal amplification and detection of SARS-CoV-2, including with crude enzymes

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About: High-surety isothermal amplification and detection of SARS-CoV-2, including with crude enzymes Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	High-surety isothermal amplification and detection of SARS-CoV-2, including with crude enzymes
Creator	Bhadra, Sanchita Ellington, Andrew Riedel, Timothy Lakhotia, Simren Tran, Nicholas
source	BioRxiv
abstract	Isothermal nucleic acid amplification tests (iNAT), such as loop-mediated isothermal amplification (LAMP), are good alternatives to polymerase chain reaction (PCR)-based amplification assays, especially for point-of-care and low resource use, in part because they can be carried out with relatively simple instrumentation. However, iNATs can generate spurious amplicons, especially in the absence of target sequences, resulting in false positive results. This is especially true if signals are based on non-sequence-specific probes, such as intercalating dyes or pH changes. In addition, pathogens often prove to be moving, evolving targets, and can accumulate mutations that will lead to inefficient primer binding and thus false negative results. Internally redundant assays targeting different regions of the target sequence can help to reduce such false negatives. Here we describe rapid conversion of three previously described SARS-CoV-2 LAMP assays that relied on non-sequence-specific readout into assays that can be visually read using sequence-specific fluorogenic oligonucleotide strand exchange (OSD) probes. We evaluate one-pot operation of both individual and multiplex LAMP-OSD assays and demonstrate detection of SARS-CoV-2 virions in crude human saliva.
has issue date	2020-07-07(xsd:dateTime)
bibo:doi	10.1101/2020.04.13.039941
has license	biorxiv
sha1sum (hex)	6548f56d0d109e8dec19397c787196d3a2c145c5
schema:url	https://doi.org/10.1101/2020.04.13.039941
resource representing a document's title	High-surety isothermal amplification and detection of SARS-CoV-2, including with crude enzymes
schema:publication	bioRxiv
resource representing a document's body	covid:6548f56d0d109e8dec19397c787196d3a2c145c5#body_text
is schema:about of	named entity 'SARS-CoV-2' named entity 'PCR' named entity 'OSD' named entity 'pathogens' named entity 'conversion' »more»

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