About: Development and evaluation of a novel high-throughput image-based fluorescent neutralization test for detection of Zika virus infection

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About: Development and evaluation of a novel high-throughput image-based fluorescent neutralization test for detection of Zika virus infection Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Development and evaluation of a novel high-throughput image-based fluorescent neutralization test for detection of Zika virus infection
Creator	Comach, Guillermo Zanluca, Camila Camacho, Daria Duarte, Claudia Koishi, Andrea »more»
source	PMC
abstract	Zika virus (ZIKV) is an emerging arbovirus belonging to the genus flavivirus that comprises other important public health viruses, such as dengue (DENV) and yellow fever (YFV). In general, ZIKV infection is a self-limiting disease, however cases of Guillain-Barré syndrome and congenital brain abnormalities in newborn infants have been reported. Diagnosing ZIKV infection remains a challenge, as viral RNA detection is only applicable until a few days after the onset of symptoms. After that, serological tests must be applied, and, as expected, high cross-reactivity between ZIKV and other flavivirus serology is observed. Plaque reduction neutralization test (PRNT) is indicated to confirm positive samples for being more specific, however it is laborious intensive and time consuming, representing a major bottleneck for patient diagnosis. To overcome this limitation, we developed a high-throughput image-based fluorescent neutralization test for ZIKV infection by serological detection. Using 226 human specimens, we showed that the new test presented higher throughput than traditional PRNT, maintaining the correlation between results. Furthermore, when tested with dengue virus samples, it showed 50.53% less cross reactivity than MAC-ELISA. This fluorescent neutralization test could be used for clinical diagnosis confirmation of ZIKV infection, as well as for vaccine clinical trials and seroprevalence studies.
has issue date	2018-03-15(xsd:dateTime)
bibo:doi	10.1371/journal.pntd.0006342
bibo:pmid	29543803
has license	cc-by
sha1sum (hex)	61022af06d0f830787f610e94f66ca49343c3de2
schema:url	https://doi.org/10.1371/journal.pntd.0006342
resource representing a document's title	Development and evaluation of a novel high-throughput image-based fluorescent neutralization test for detection of Zika virus infection
has PubMed Central identifier	PMC5871014
has PubMed identifier	29543803
schema:publication	PLoS Negl Trop Dis
resource representing a document's body	covid:61022af06d0f830787f610e94f66ca49343c3de2#body_text
is schema:about of	named entity 'fluorescent' named entity 'confirmation' named entity 'cross reactivity' named entity 'seroprevalence' named entity 'diagnosis' »more»

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