About: Development and evaluation of loop‐mediated isothermal amplification assay for rapid and sensitive detection of canine parvovirus DNA directly in faecal specimens

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About: Development and evaluation of loop‐mediated isothermal amplification assay for rapid and sensitive detection of canine parvovirus DNA directly in faecal specimens Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Development and evaluation of loop‐mediated isothermal amplification assay for rapid and sensitive detection of canine parvovirus DNA directly in faecal specimens
Creator	Pillai, R Amsaveni, S Antony, P Matta, S Mukhopadhyay, H »more»
source	Medline; PMC
abstract	Aims: To develop a specific and highly sensitive loop‐mediated isothermal amplification (LAMP) technique for the rapid detection of canine parvovirus (CPV) DNA directly in suspected faecal samples of dogs by employing a simple method of template preparation. Methods and Results: LAMP reaction was developed by designing two sets of outer and inner primers, which target a total of six distinct regions on VP2 gene of CPV. The template DNA was prepared by a simple boiling and chilling method. Of the 140 faecal samples screened by the developed LAMP and the conventional PCR assays, 104 samples (74·28%) were found positive by LAMP, whereas 81 samples (57·85%) were found positive by PCR. The specificity of the LAMP assay was tested by cross‐examination of common pathogens of dogs and further confirmed by sequencing. The detection limit of the LAMP was 0·0001 TCID(50) ml(−1), whereas the detection limit of the PCR was 1000 TCID(50) ml(−1). Conclusions: The developed LAMP assay detects CPV DNA in faecal specimens directly within an hour by following a simple and rapid boiling and chilling method of template preparation. The result also shows that the developed LAMP assay is specific and highly sensitive in detecting CPV. Significance and Impact of the Study: The result indicates the potential usefulness of LAMP which is a simple, rapid, specific, highly sensitive and cost‐effective field‐based method for direct detection of CPV from the suspected faecal samples of dogs.
has issue date	2012-07-24(xsd:dateTime)
bibo:doi	10.1111/j.1472-765x.2012.03284.x
bibo:pmid	22748120
has license	no-cc
sha1sum (hex)	5c93ae8126c344f27489797a64e02c2c514d0de6
schema:url	https://doi.org/10.1111/j.1472-765x.2012.03284.x
resource representing a document's title	Development and evaluation of loop‐mediated isothermal amplification assay for rapid and sensitive detection of canine parvovirus DNA directly in faecal specimens
has PubMed Central identifier	PMC7197762
has PubMed identifier	22748120
schema:publication	Lett Appl Microbiol
resource representing a document's body	covid:5c93ae8126c344f27489797a64e02c2c514d0de6#body_text
is schema:about of	named entity 'loop-mediated isothermal amplification' named entity 'parvovirus' named entity 'evaluation' named entity 'assay' named entity 'canine parvovirus' »more»

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