About: Generation and validation of a highly sensitive bioluminescent HIV-1 reporter vector that simplifies measurement of virus release

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About: Generation and validation of a highly sensitive bioluminescent HIV-1 reporter vector that simplifies measurement of virus release Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Generation and validation of a highly sensitive bioluminescent HIV-1 reporter vector that simplifies measurement of virus release
Creator	Kirui, Freed Retrovirology,
source	PMC
abstract	BACKGROUND: The continued persistence of HIV-1 as a public health concern due to the lack of a cure calls for the development of new tools for studying replication of the virus. Here, we used NanoLuc, a small and extremely bright luciferase protein, to develop an HIV-1 bioluminescent reporter virus that simplifies functional measurement of virus particle production. RESULTS: The reporter virus encodes a Gag protein containing NanoLuc inserted between the matrix (MA) and capsid (CA) domains of Gag, thereby generating virus particles that package high levels of the NanoLuc reporter. We observe that inserting the NanoLuc protein within HIV-1 Gag has minimal impact on Gag expression and virus particle release. We show that the reporter virus recapitulates inhibition of HIV-1 particle release by Gag mutations, the restriction factor tetherin, and the small-molecule inhibitor amphotericin-B methyl ester. CONCLUSION: These results demonstrate that this vector will provide a simple and rapid tool for functional studies of virus particle assembly and release and high-throughput screening for cellular factors and small molecules that promote or inhibit HIV-1 particle production.
has issue date	2020-05-19(xsd:dateTime)
bibo:doi	10.1186/s12977-020-00521-5
bibo:pmid	32430080
has license	cc-by
sha1sum (hex)	5c5524493ed748f8f749ca494fb2b7a42a246410
schema:url	https://doi.org/10.1186/s12977-020-00521-5
resource representing a document's title	Generation and validation of a highly sensitive bioluminescent HIV-1 reporter vector that simplifies measurement of virus release
has PubMed Central identifier	PMC7235552
has PubMed identifier	32430080
schema:publication	Retrovirology
resource representing a document's body	covid:5c5524493ed748f8f749ca494fb2b7a42a246410#body_text
is schema:about of	named entity 'luciferase' named entity 'HIV-1' named entity 'development' named entity 'p24 antigen' named entity 'protein' »more»

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