About: First environmental surveillance for the presence of SARS-CoV-2 RNA in wastewater and river water in Japan

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About: First environmental surveillance for the presence of SARS-CoV-2 RNA in wastewater and river water in Japan Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	First environmental surveillance for the presence of SARS-CoV-2 RNA in wastewater and river water in Japan
Creator	Kitajima, Masaaki Haramoto, Eiji Malla, Bikash
source	MedRxiv
abstract	Wastewater-based epidemiology can be a powerful tool to understand the actual incidence of coronavirus disease 2019 (COVID-19) in a community because severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the etiological agent of COVID-19, can be shed in the feces of infected individuals regardless of their symptoms. The present study aimed to assess the presence of SARS-CoV-2 RNA in wastewater and river water in Yamanashi Prefecture, Japan, using four quantitative and two nested PCR assays. Influent and secondary-treated (before chlorination) wastewater samples and river water samples were collected five times from a wastewater treatment plant and three times from a river, respectively, between March 17 and May 7, 2020. The wastewater and river water samples (200-5,000 mL) were processed by using two different methods: the electronegative membrane-vortex (EMV) method and the membrane adsorption-direct RNA extraction method. Based on the observed concentrations of indigenous pepper mild mottle virus RNA, the EMV method was found superior to the membrane adsorption-direct RNA extraction method. SARS-CoV-2 RNA was successfully detected in one of five secondary-treated wastewater samples with a concentration of 2.4 x 103 copies/L by N_Sarbeco qPCR assay following the EMV method, whereas all the influent samples were tested negative for SARS-CoV-2 RNA. This result could be attributed to higher limit of detection for influent (4.0 x 103-8.2 x 104 copies/L) with a lower filtration volume (200 mL) compared to that for secondary-treated wastewater (1.4 x 102-2.5 x 103 copies/L) with a higher filtration volume of 5,000 mL. None of the river water samples tested positive for SARS-CoV-2 RNA. Comparison with the reported COVID-19 cases in Yamanashi Prefecture showed that SARS-CoV-2 RNA was detected in the secondary-treated wastewater sample when the cases peaked in the community. This is the first study reporting the detection of SARS-CoV-2 RNA in wastewater in Japan.
has issue date	2020-06-07(xsd:dateTime)
bibo:doi	10.1101/2020.06.04.20122747
has license	medrxiv
sha1sum (hex)	5b052d0a3c5a09928dd1643a9891090a390dca01
schema:url	https://doi.org/10.1101/2020.06.04.20122747
resource representing a document's title	First environmental surveillance for the presence of SARS-CoV-2 RNA in wastewater and river water in Japan
resource representing a document's body	covid:5b052d0a3c5a09928dd1643a9891090a390dca01#body_text
is schema:about of	named entity 'SARS-COV-2 RNA' named entity 'DETECTED' named entity 'QUANTITATIVE' named entity 'CONCENTRATION OF' named entity 'FILTRATION' »more»

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