About: Simultaneous Determination of Four Active Components in Tobacco Wastes by LC

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Simultaneous Determination of Four Active Components in Tobacco Wastes by LC
Creator	Lu, Ding-Qiang Ouyang, Ping-Kai Wang, Jun Wang, Jia-Li Ling, Xiu-Quan »more»
source	PMC
abstract	A liquid chromatographic method was developed for the simultaneous quantification of four major active components in tobacco (Nicotiana tobaccum L.) wastes. Samples were extracted with 70% v/v aqueous methanol, four compounds including chlorogenic acid, cryptochlorogenic acid, neochlorogenic acid and caffeic acid were identified and determined by using LC coupled to electrospray tandem mass spectrometry and LC–UV method, respectively. Separation in LC–UV was on an Alltima C(18) column (250 mm × 4.6 mm i.d.; 5 μm) with a mobile phase consisting acetonitrile: ammonium acetate buffer (pH 4.5) (5:95 v/v), at a flow rate of 1.0 mL min(−1), detected at 327 nm. Four regression equations showed good linear relationships (r (2) > 0.999) between the peak area of each marker and concentration. The method has good repeatability and precision, the intra-day and inter-day RSD for both retention time and peak area was less than 1.0%. The recoveries, measured at three concentration levels, varied from 96.33 to 101.10%. The LOD (S/N = 3) and LOQ (S/N = 6) were less than 0.010 and 0.795 μg·mL(−1), respectively. This assay was successfully applied to the determination of four active compounds in ten samples. The results indicated that the developed assay method was rapid, accurate, reliable and could be readily utilized as a quantitative analysis method for various of tobacco wastes.
has issue date	2008-12-12(xsd:dateTime)
bibo:doi	10.1365/s10337-008-0908-8
bibo:pmid	32214427
has license	no-cc
sha1sum (hex)	57c290e6856e6e2a359b0cd928178ddda6a2332f
schema:url	https://doi.org/10.1365/s10337-008-0908-8
resource representing a document's title	Simultaneous Determination of Four Active Components in Tobacco Wastes by LC
has PubMed Central identifier	PMC7087915
has PubMed identifier	32214427
schema:publication	Chromatographia
resource representing a document's body	covid:57c290e6856e6e2a359b0cd928178ddda6a2332f#body_text
is schema:about of	named entity 'measured' named entity 'Samples' named entity 'S/N' named entity 'i.d.' named entity 'ACETONITRILE' »more»

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