About: Proteolytic Disassembly Is a Critical Determinant for Reovirus Oncolysis

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Proteolytic Disassembly Is a Critical Determinant for Reovirus Oncolysis
Creator	Forsyth, Peter Alain, Tommy Liacini, Adelhamid Lun, Xueqing Schiff, Leslie »more»
source	Elsevier; Medline; PMC
abstract	See page 1406 Mammalian ortheoreoviruses are currently being investigated as novel cancer therapeutics, but the cellular mechanisms that regulate susceptibility to reovirus oncolysis remain poorly understood. In this study, we present evidence that virion disassembly is a key determinant of reovirus oncolysis. To penetrate cell membranes and initiate infection, the outermost capsid proteins of reovirus must be proteolyzed to generate a disassembled particle called an infectious subviral particle (ISVP). In fibroblasts, this process is mediated by the endo/lysosomal proteases cathepsins B and L. We have analyzed the early events of infection in reovirus-susceptible and -resistant cells. We find that, in contrast to susceptible glioma cells and Ras-transformed NIH3T3 cells, reovirus-resistant cancer cells and untransformed NIH3T3 cells restrict virion uncoating and subsequent gene expression. Disassembly-restrictive cells support reovirus infection, as in vitro-generated ISVPs establish productive infection, and pretreatment with poly(I:C) does not prevent infection in cancer cells. We find that the level of active cathepsin B and L is increased in tumors and that disassembly-restrictive glioma cells support reovirus oncolysis when grown as a tumor in vivo. Together, these results provide a model in which proteolytic disassembly of reovirus is a critical determinant of susceptibility to reovirus oncolysis.
has issue date	2016-12-14(xsd:dateTime)
bibo:doi	10.1038/sj.mt.6300207
bibo:pmid	17519890
has license	no-cc
sha1sum (hex)	572f946157035c9ae35e6b911552e015b4597318
schema:url	https://doi.org/10.1038/sj.mt.6300207
resource representing a document's title	Proteolytic Disassembly Is a Critical Determinant for Reovirus Oncolysis
has PubMed Central identifier	PMC7185731
has PubMed identifier	17519890
schema:publication	Mol Ther
resource representing a document's body	covid:572f946157035c9ae35e6b911552e015b4597318#body_text
is schema:about of	named entity 'oncolysis' named entity 'regulate' named entity 'reovirus' named entity 'provide' named entity 'subsequent' »more»

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