About: Optimized qRT-PCR Approach for the Detection of Intra- and Extra-Cellular SARS-CoV-2 RNAs

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About: Optimized qRT-PCR Approach for the Detection of Intra- and Extra-Cellular SARS-CoV-2 RNAs Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Optimized qRT-PCR Approach for the Detection of Intra- and Extra-Cellular SARS-CoV-2 RNAs
Creator	Berger, Annemarie Ciesek, Sandra Bojkova, Denisa Cinatl, Jindrich Hoehl, Sebastian »more»
source	Medline; PMC
abstract	The novel coronavirus SARS-CoV-2 is the causative agent of the acute respiratory disease COVID-19, which has become a global concern due to its rapid spread. Meanwhile, increased demand for testing has led to a shortage of reagents and supplies and compromised the performance of diagnostic laboratories in many countries. Both the World Health Organization (WHO) and the Center for Disease Control and Prevention (CDC) recommend multi-step RT-PCR assays using multiple primer and probe pairs, which might complicate the interpretation of the test results, especially for borderline cases. In this study, we describe an alternative RT-PCR approach for the detection of SARS-CoV-2 RNA that can be used for the probe-based detection of clinical isolates in diagnostics as well as in research labs using a low-cost SYBR green method. For the evaluation, we used samples from patients with confirmed SARS-CoV-2 infections and performed RT-PCR assays along with successive dilutions of RNA standards to determine the limit of detection. We identified an M-gene binding primer and probe pair highly suitable for the quantitative detection of SARS-CoV-2 RNA for diagnostic and research purposes.
has issue date	2020-06-20(xsd:dateTime)
bibo:doi	10.3390/ijms21124396
bibo:pmid	32575728
has license	cc-by
sha1sum (hex)	4bf14bddd7478d8ac9d63b288e86fb0960023c80
schema:url	https://doi.org/10.3390/ijms21124396
resource representing a document's title	Optimized qRT-PCR Approach for the Detection of Intra- and Extra-Cellular SARS-CoV-2 RNAs
has PubMed Central identifier	PMC7352576
has PubMed identifier	32575728
schema:publication	Int J Mol Sci
resource representing a document's body	covid:4bf14bddd7478d8ac9d63b288e86fb0960023c80#body_text
is schema:about of	named entity 'RT-PCR' named entity 'labs' named entity 'For' named entity 'RT-PCR' named entity 'COVID-19' »more»

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