About: EIF3i affects vesicular stomatitis virus growth by interacting with matrix protein

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	EIF3i affects vesicular stomatitis virus growth by interacting with matrix protein
Creator	Gao, Feng He, Wenqi Lan, Yungang Lu, Huijun Song, Deguang »more»
source	Elsevier; Medline; PMC
abstract	The matrix protein of vesicular stomatitis virus (VSV) performs multiple functions during viral genome replication and virion production and is involved in modulating multiple host signaling pathways that favor virus replication. To perform numerous functions within infected cells, the M protein needs to recruit cellular partners. To better understand the role of M during VSV replication, we looked for interacting partners by using the two-hybrid system. The eukaryotic translation initiation factor 3, subunit i (eIF3i) was identified to be an M-binding partner, and this interaction was validated by GST pull-down and laser confocal assays. Through a mutagenesis analysis, we found that some mutants of M between amino acids 122 and 181 impaired but did not completely abolish the M–eIF3i interaction. Furthermore, the knockdown of eIF3i by RNA interference decreased viral replication and transcription in the early stages but led to increase in later stages. VSV transcription was increased at 4 h post-infection but was not changed at 8 and 12 h post-infection after the over-expression of eIF3i. Finally, we also demonstrated that VSV could inhibit the activity of Akt1 and that the knockdown of eIF3i inhibited the expression of the ISGs regulated by phospho-Akt1. These results indicated that eIF3i may affect VSV growth by regulating the host antiviral response in HeLa cells.
has issue date	2017-11-07(xsd:dateTime)
bibo:doi	10.1016/j.vetmic.2017.10.021
bibo:pmid	29173589
has license	no-cc
sha1sum (hex)	4942c524b2d3ef76f18d3a08b30981e0a4820939
schema:url	https://doi.org/10.1016/j.vetmic.2017.10.021
resource representing a document's title	EIF3i affects vesicular stomatitis virus growth by interacting with matrix protein
has PubMed Central identifier	PMC7117458
has PubMed identifier	29173589
schema:publication	Vet Microbiol
resource representing a document's body	covid:4942c524b2d3ef76f18d3a08b30981e0a4820939#body_text
is schema:about of	named entity 'regulating' named entity 'antiviral' named entity 'VSV' named entity 'recruit' named entity 'response' »more»

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