About: A potent neutralizing nanobody against SARS-CoV-2 with inhaled delivery potential

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About: A potent neutralizing nanobody against SARS-CoV-2 with inhaled delivery potential Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	A potent neutralizing nanobody against SARS-CoV-2 with inhaled delivery potential
Creator	Gong, Rui Zhang, Yanmin Zhu, Min Ma, Linlin Zhang, Hao »more»
source	BioRxiv
abstract	The outbreak of COVID-19 has emerged as a global pandemic. The unprecedented scale and severity call for rapid development of effective prophylactics or therapeutics. We here reported Nanobody (Nb) phage display libraries derived from four camels immunized with the SARS-CoV-2 spike receptor-binding domain (RBD), from which 381 Nbs were identified to recognize SARS-CoV-2-RBD. Furthermore, seven Nbs were shown to block interaction of human angiotensin converting enzyme 2 (ACE2) with SARS-CoV-2-RBD-variants, bat-SL-CoV-WIV1-RBD and SARS-CoV-1-RBD. Among the seven candidates, Nb11-59 exhibited the highest activity against authentic SARS-CoV-2 with ND50 of 0.55 μg/mL. Nb11-59 can be produced on a large-scale in Pichia pastoris, with 20 g/L titer and 99.36% purity. It also showed good stability profile, and nebulization did not impact its stability. Overall, Nb11-59 might be a promising prophylactic and therapeutic molecule against COVID-19, especially through inhalation delivery. Graphical Abstract
has issue date	2020-08-14(xsd:dateTime)
bibo:doi	10.1101/2020.08.09.242867
has license	biorxiv
sha1sum (hex)	46b3891ebdc06bf9dd1d1111932c63ea0586f7e7
schema:url	https://doi.org/10.1101/2020.08.09.242867
resource representing a document's title	A potent neutralizing nanobody against SARS-CoV-2 with inhaled delivery potential
schema:publication	bioRxiv
resource representing a document's body	covid:46b3891ebdc06bf9dd1d1111932c63ea0586f7e7#body_text
is schema:about of	named entity 'activity' named entity 'spike' named entity 'RBD' named entity 'SARS-CoV-2' named entity 'Pichia pastoris' »more»

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