About: Comparison of commercial systems for extraction of nucleic acids from DNA/RNA respiratory pathogens

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Comparison of commercial systems for extraction of nucleic acids from DNA/RNA respiratory pathogens
Creator	Erdman, Dean Yang, Genyan Kodani, Maja Kools, John Fields, Barry »more»
source	Elsevier; Medline; PMC
abstract	Abstract This study compared six automated nucleic acid extraction systems and one manual kit for their ability to recover nucleic acids from human nasal wash specimens spiked with five respiratory pathogens, representing Gram-positive bacteria (Streptococcus pyogenes), Gram-negative bacteria (Legionella pneumophila), DNA viruses (adenovirus), segmented RNA viruses (human influenza virus A), and non-segmented RNA viruses (respiratory syncytial virus). The robots and kit evaluated represent major commercially available methods that are capable of simultaneous extraction of DNA and RNA from respiratory specimens, and included platforms based on magnetic-bead technology (KingFisher mL, Biorobot EZ1, easyMAG, KingFisher Flex, and MagNA Pure Compact) or glass fiber filter technology (Biorobot MDX and the manual kit Allprep). All methods yielded extracts free of cross-contamination and RT-PCR inhibition. All automated systems recovered L. pneumophila and adenovirus DNA equivalently. However, the MagNA Pure protocol demonstrated more than 4-fold higher DNA recovery from the S. pyogenes than other methods. The KingFisher mL and easyMAG protocols provided 1- to 3-log wider linearity and extracted 3- to 4-fold more RNA from the human influenza virus and respiratory syncytial virus. These findings suggest that systems differed in nucleic acid recovery, reproducibility, and linearity in a pathogen specific manner.
has issue date	2011-01-31(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2010.10.024
bibo:pmid	21034773
has license	els-covid
sha1sum (hex)	45d99cedfe05f8ead1ed59c03df421307214cd74
schema:url	https://doi.org/10.1016/j.jviromet.2010.10.024
resource representing a document's title	Comparison of commercial systems for extraction of nucleic acids from DNA/RNA respiratory pathogens
has PubMed Central identifier	PMC7112907
has PubMed identifier	21034773
schema:publication	Journal of Virological Methods
resource representing a document's body	covid:45d99cedfe05f8ead1ed59c03df421307214cd74#body_text
is schema:about of	named entity 'All' named entity 'simultaneous' named entity 'segmented' named entity 'However' named entity 'manual' »more»

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