About: Expression of the human immunodeficiency virus frameshift signal in a bacterial cell-free system: Influence of an interaction between the ribosome and a stem-loop structure downstream from the slippery site

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About: Expression of the human immunodeficiency virus frameshift signal in a bacterial cell-free system: Influence of an interaction between the ribosome and a stem-loop structure downstream from the slippery site Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Expression of the human immunodeficiency virus frameshift signal in a bacterial cell-free system: Influence of an interaction between the ribosome and a stem-loop structure downstream from the slippery site
Creator	Brakier-Gingras, Léa Brunelle, Marie-Noëlle Lemay, Guy Payant, Catherine
source	Medline; PMC
abstract	A -1 frameshift event is required for expression of the pol gene when ribosomes translate the mRNA of human immunodeficiency virus type-1 (HIV-1). In this study, we inserted the frameshift region of HIV-1 (a slippery heptanucleotide motif followed by a stem-loop) in a reporter gene coding for firefly luciferase. The ability of the corresponding mRNA, generated by in vitro transcription, to be translated in an Escherichia coli cell-free extract is the first demonstration that the HIV-1 frameshift can be reproduced in a bacterial cell-free extract, providing a powerful approach for analysis of the frameshift mechanism. The responses of the frameshift signal to chloramphenicol, an inhibitor of peptide bond formation, and spectinomycin, an inhibitor of translocation, suggest that the frameshift complies with the same rules found in eukaryotic translation systems. Furthermore, when translation was performed in the presence of streptomycin and neamine, two errorinducing antibiotics, or with hyperaccurate ribosomes mutated in S12, the frameshift efficiency was increased or decreased, respectively, but only in the presence of the stem-loop, suggesting that the stem-loop can influence the frameshift through a functional interaction with the ribosomes.
has issue date	1999-12-15(xsd:dateTime)
bibo:doi	10.1093/nar/27.24.4783
bibo:pmid	10572179
has license	green-oa
sha1sum (hex)	41e5c4742cbe73246e7488fc1e52bf5552541e19
schema:url	https://doi.org/10.1093/nar/27.24.4783
resource representing a document's title	Expression of the human immunodeficiency virus frameshift signal in a bacterial cell-free system: Influence of an interaction between the ribosome and a stem-loop structure downstream from the slippery site
has PubMed Central identifier	PMC148779
has PubMed identifier	10572179
schema:publication	Nucleic Acids Research
resource representing a document's body	covid:41e5c4742cbe73246e7488fc1e52bf5552541e19#body_text
is schema:about of	named entity 'ribosomes' named entity 'influence' named entity 'HIV-1' named entity 'eukaryotic translation' named entity 'INTERACTION' »more»

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