About: Attenuated SARS-CoV-2 variants with deletions at the S1/S2 junction

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About: Attenuated SARS-CoV-2 variants with deletions at the S1/S2 junction Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Attenuated SARS-CoV-2 variants with deletions at the S1/S2 junction
Creator	Chan, Jasper Fuk-Woo Yuen, Kwok-Yung Chen, Zhiwei Chan, Kwok-Hung Chu, Hin »more»
source	Medline; PMC
abstract	The emergence of SARS-CoV-2 has led to the current global coronavirus pandemic and more than one million infections since December 2019. The exact origin of SARS-CoV-2 remains elusive, but the presence of a distinct motif in the S1/S2 junction region suggests the possible acquisition of cleavage site(s) in the spike protein that promoted cross-species transmission. Through plaque purification of Vero-E6 cultured SARS-CoV-2, we found a series of variants which contain 15-30-bp deletions (Del-mut) or point mutations respectively at the S1/S2 junction. Examination of the original clinical specimen from which the isolate was derived, and 26 additional SARS-CoV-2 positive clinical specimens, failed to detect these variants. Infection of hamsters shows that one of the variants (Del-mut-1) which carries deletion of 10 amino acids (30bp) does not cause the body weight loss or more severe pathological changes in the lungs that is associated with wild type virus infection. We suggest that the unique cleavage motif promoting SARS-CoV-2 infection in humans may be under strong selective pressure, given that replication in permissive Vero-E6 cells leads to the loss of this adaptive function. It would be important to screen the prevalence of these variants in asymptomatic infected cases. The potential of the Del-mut variants as an attenuated vaccine or laboratory tool should be evaluated.
has issue date	2020-05-04(xsd:dateTime)
bibo:doi	10.1080/22221751.2020.1756700
bibo:pmid	32301390
has license	cc-by
sha1sum (hex)	3d899b8ad978035c4435528bbd2b7695e5b3195b
schema:url	https://doi.org/10.1080/22221751.2020.1756700
resource representing a document's title	Attenuated SARS-CoV-2 variants with deletions at the S1/S2 junction
has PubMed Central identifier	PMC7241555
has PubMed identifier	32301390
schema:publication	Emerg Microbes Infect
resource representing a document's body	covid:3d899b8ad978035c4435528bbd2b7695e5b3195b#body_text
is schema:about of	named entity 'cleavage' named entity 'cultured' named entity 'origin' named entity 'deletions' named entity 'humans' »more»

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