About: The Y271 and I274 Amino Acids in Reverse Transcriptase of Human Immunodeficiency Virus-1 Are Critical to Protein Stability

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About: The Y271 and I274 Amino Acids in Reverse Transcriptase of Human Immunodeficiency Virus-1 Are Critical to Protein Stability Goto Sponge NotDistinct Permalink

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	The Y271 and I274 Amino Acids in Reverse Transcriptase of Human Immunodeficiency Virus-1 Are Critical to Protein Stability
Creator	Zheng, Bo-Jian Jin, Dong-Yan Wu, Hao Wen, Yu-Mei Zhang, Hao-Jie »more»
source	PMC
abstract	Reverse transcriptase (RT) of human immunodeficiency virus (HIV)-1 plays a key role in initiating viral replication and is an important target for developing anti-HIV drugs. Our previous study showed that two mutations (Y271A and I274A) in the turn RT (Gln(269)-Arg(277)) abrogated viral replication, but the replication capacity and RT activity was discordant. In this study, we further investigated why alanine substitutions at these two sites would affect viral replication. We found that both RT activity and RT protein were almost undetectable in viral particles of these two mutants, although the Pr160(gag-pol) mutants were properly expressed, transported and incorporated. Using protease inhibition assay, we demonstrated a correlation between the degradation of the RT mutants and the activity of viral protease. Our native gel analysis indicated that the mutations at 271 and 274 amino acids might cause conformational changes, leading to the formation of higher order oligomers instead of dimers, resulting in increased protein instability and susceptibility to viral protease. Thus, residues 271 and 274 are critical to RT stability and resistance to viral protease. The conservation of the two amino acid residues among different strains of HIV-1 lent further support to this conclusion. The knowledge gained here may prove useful in drug design.
has issue date	2009-07-03(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0006108
bibo:pmid	19578544
has license	cc-by
sha1sum (hex)	3576b9e0d1895a2d2cb0231304425e1633e86e82
schema:url	https://doi.org/10.1371/journal.pone.0006108
resource representing a document's title	The Y271 and I274 Amino Acids in Reverse Transcriptase of Human Immunodeficiency Virus-1 Are Critical to Protein Stability
has PubMed Central identifier	PMC2701634
has PubMed identifier	19578544
schema:publication	PLoS One
resource representing a document's body	covid:3576b9e0d1895a2d2cb0231304425e1633e86e82#body_text
is schema:about of	named entity 'correlation' named entity 'Our' named entity 'investigated' named entity 'The knowledge' named entity 'REVERSE TRANSCRIPTASE' »more»

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