About: Development and characterization of 20 polymorphic microsatellite markers for the white-bellied pangolin Phataginus tricuspis (Mammalia, Pholidota)

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About: Development and characterization of 20 polymorphic microsatellite markers for the white-bellied pangolin Phataginus tricuspis (Mammalia, Pholidota) Goto Sponge NotDistinct Permalink

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Development and characterization of 20 polymorphic microsatellite markers for the white-bellied pangolin Phataginus tricuspis (Mammalia, Pholidota)
Creator	Aguillon, Samantha Din Dipita, Alain Lecompte, Emilie Tindo, Maurice Didier Missoup, Alain »more»
source	Medline; PMC
abstract	Pangolins, or scaly anteaters, have recently been flagshiped as one of the most illegally traded mammals, and as a corollary, as potential intermediate hosts at the origin of the COVID-19 pandemic. In order to improve the traceability of their trade, we developed 20 polymorphic microsatellite loci for the white-bellied pangolin (Phataginus tricuspis), the species most frequently found on African bushmeat markets. We genotyped 24 white-bellied pangolins from the Douala market, Cameroon, originating from the Ebo forest c. 75 km north-east of Douala. The number of alleles per locus ranged from 4 to 12 (mean = 6.95), and mean observed and expected heterozygosities were 0.592 (0.208–0.875) and 0.671 (0.469–0.836), respectively. Genetic diversity was higher than that cross-estimated from microsatellite loci developed for other species of pangolins. Two loci deviated from Hardy–Weinberg equilibrium and two loci showed linkage disequilibrium. Genetic variance (PCoA) was increased with the addition of 13 pangolins of unknown origin, possibly suggesting that the Douala market is fed from differentiated source populations of white-bellied pangolins. Each of the 37 individuals had a unique multilocus genotype. The unbiased probability of identity (uPI) and the probability of identity among siblings (PIsibs) were both very low (uPI = 8.443 e−21; PIsibs = 1.011 e−07). Only five microsatellite loci were needed to reach the conservative value of PIsibs < 0.01, overall indicating a powerful discriminating power of our combined loci. These 20 newly developed microsatellite loci might prove useful in tracing the local-to-global trade of the white-bellied pangolin, and will hopefully contribute to the DNA-assisted implementation of future conservation strategies at reasonable costs. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s11033-020-05511-6) contains supplementary material, which is available to authorized users.
has issue date	2020-05-17(xsd:dateTime)
bibo:doi	10.1007/s11033-020-05511-6
bibo:pmid	32419053
has license	no-cc
sha1sum (hex)	349f9d6b38bda8402d0af58bb8167d2572084e78
schema:url	https://doi.org/10.1007/s11033-020-05511-6
resource representing a document's title	Development and characterization of 20 polymorphic microsatellite markers for the white-bellied pangolin Phataginus tricuspis (Mammalia, Pholidota)
has PubMed Central identifier	PMC7230135
has PubMed identifier	32419053
schema:publication	Mol Biol Rep
resource representing a document's body	covid:349f9d6b38bda8402d0af58bb8167d2572084e78#body_text
is schema:about of	named entity 'developed' named entity 'Each' named entity 'alleles' named entity 'loci' named entity 'Genetic diversity' »more»

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