About: Silence of TGF-β1 gene expression reduces prrsv replication and potentiates immunity of immune cells of tibetan pig

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Silence of TGF-β1 gene expression reduces prrsv replication and potentiates immunity of immune cells of tibetan pig Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Silence of TGF-β1 gene expression reduces prrsv replication and potentiates immunity of immune cells of tibetan pig
Creator	Gao, Rong Wang, Ye Chen, Yihui Wang, Zezhou Chen, Xiao-Hui »more»
source	PMC
abstract	Transforming growth factor beta 1 (TGF-β1) was of importance in the pathogenesis of porcine reproductive and respiratory syndrome virus (PRRSV). To determine whether knockdown of TGF-β1 gene expression could facilitate the control of PRRSV infection, specific sequences for expressing shRNA targeted to porcine TGF-β1 gene were synthesized and cloned into pSilencer 3.1-H1 neovector. Then they were used to transfect peripheral blood mononuclear cells of Tibetan pig (Tp-PBMCs) followed by PRRSV inoculation. The positive recombinant plasmids were screened for inhibition of TGF-β1 gene expression by real-time quantitative RT-PCR. Conversely, the mRNA level of PRRSV in shRNA treated Tp-PBMCs dramatically decreased, and there were significant increases of the transcription of immune genes, such as interleukin-2 (IL-2), interleukin-4 (IL-4), interferon-alpha (IFN-α), interferon-gamma (IFN-γ), tumor necrosis factor-alpha (TNF-α), toll-like receptor 3 (TLR3), toll-like receptor 7 (TLR7), Myeloid differentiation primary response gene (88) (MyD88), and interleukin-27p28 (IL-27p28). However, the expressions of IL-8 and IL-10 genes significantly reduced in comparison to the control infected cells. In addition, transfection with the shRNA plasmids significantly elevated the viability of immune cells. Therefore the knockdown of TGF-β1 gene expression by shRNA not only inhibits the replication of PRRSV but also improves immune responsiveness following viral infection, suggesting a novel way to facilitate the control of PRRSV infection in pigs.
has issue date	2019-09-26(xsd:dateTime)
bibo:doi	10.1016/j.vas.2019.100074
has license	cc-by-nc-nd
sha1sum (hex)	22ad578f8024dd9d3265753b238a660d787acb18
schema:url	https://doi.org/10.1016/j.vas.2019.100074
resource representing a document's title	Silence of TGF-β1 gene expression reduces prrsv replication and potentiates immunity of immune cells of tibetan pig
has PubMed Central identifier	PMC7386707
schema:publication	Vet Anim Sci
resource representing a document's body	covid:22ad578f8024dd9d3265753b238a660d787acb18#body_text
is schema:about of	named entity 'real-time' named entity 'TLR3' named entity 'interferon-gamma' named entity 'IFN-α' named entity 'interferon-alpha' »more»

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software