About: Reference gene selection for the shell gland of laying hens in response to time-points of eggshell formation and nicarbazin

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About: Reference gene selection for the shell gland of laying hens in response to time-points of eggshell formation and nicarbazin Goto Sponge NotDistinct Permalink

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Reference gene selection for the shell gland of laying hens in response to time-points of eggshell formation and nicarbazin
Creator	Roberts, Juliet Samiullah, Sami Wu, Shu-Biao
source	PMC
abstract	Ten reference genes were investigated for normalization of gene expression data in the shell gland of laying hens. Analyses performed with geNorm revealed that hypoxanthine phosphoribosyltransferase 1 (HPRT1) and hydroxymethylbilane synthase (HMBS) were the two most stable reference genes in response to post-oviposition time alone (POT) or with nicarbazin treatment (POT+N) of laying hens. NormFinder analyses showed that the two most stable reference genes in response to POT and POT+N were 18S ribosomal RNA (18S rRNA), ribosomal protein L4 (RPL4) and HMBS, RPL4, respectively. BestKeeper analyses showed that 18S rRNA, RPL4 and HPRT1, HMBS were the two most stable reference genes for POT, and POT+N, respectively. Of the ten reference genes, all except B2M showed geNorm M <0.5, suggesting that they were stably expressed in the shell gland tissue. Consensus from these three programs suggested HPRT1 and HMBS could be used as the two most stable reference genes in the present study. Expression analyses of four candidate target genes with the two most and the two least stable genes showed that a combination of stable reference genes leads to more discriminable quantification of expression levels of target genes, while the least stable genes failed to do so. Therefore, HMBS and HPRT1 are recommended as the two most stable reference genes for the normalization of gene expression data at different stages of eggshell formation in brown-egg laying hens. Available statistical programs for reference gene ranking should include more robust analysis capability to analyse the gene expression data generated from factorial design experiments.
has issue date	2017-07-03(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0180432
bibo:pmid	28671969
has license	cc-by
sha1sum (hex)	222f2de4774452963b9c8b640faf80dced073b64
schema:url	https://doi.org/10.1371/journal.pone.0180432
resource representing a document's title	Reference gene selection for the shell gland of laying hens in response to time-points of eggshell formation and nicarbazin
has PubMed Central identifier	PMC5495395
has PubMed identifier	28671969
schema:publication	PLoS One
resource representing a document's body	covid:222f2de4774452963b9c8b640faf80dced073b64#body_text
is schema:about of	named entity 'genes' named entity 'laying hens' named entity 'investigated' named entity 'target' named entity 'genes' »more»

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